Metallothionein promotes laminin-1-induced acinar differentiation in vitro and reduces tumor growth in vivo

被引:0
作者
Hecht, D [1 ]
Jung, D [1 ]
Prabhu, VV [1 ]
Munson, PJ [1 ]
Hoffman, MP [1 ]
Kleinman, HK [1 ]
机构
[1] Natl Inst Dent & Craniofacial Res, NIH, Ctr Informat Technol, Bethesda, MD 20892 USA
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中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Laminin-1 was found previously to promote the morphological differentiation of a salivary gland cell line (HSG) into acinar-like structures with polarized nuclei (1). Here, microarray analysis showed that laminin-1 induced mainly one gene family of proteins, the metal binding metallothioneins (MTs), out of more than 10,500 cDNAs screened. Northern and protein analyses demonstrated that MT was increased some 5-10-fold by laminin-1 as early as 6 It after incubation. Cells transfected with this gene formed 3-5-fold larger acinar-like structures on exposure to laminin-1 in vitro and smaller, more differentiated tumors in vivo. We conclude that MTs are important in acinar cell morphological differentiation and may have novel functions other than metal binding.
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页码:5370 / 5374
页数:5
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