Preparation of immobilized glucose oxidase column by sol-gel technique and its application to the chemiluminescent glucose sensor

Glucose oxidase (GOD) was immobilized in the course of gelation of the sol of tetraethylorthosilicate(TEOS), and immobilized GOD columns were easily prepared with different carriers (silica gel and molecular sieve) using this system. It was demonstrated that the activity of GOD remained high in sol-gel matrix. With the application of the GOD column prepared with sol-gel method to the flow injection chemiluminescent system, glucose could be effectively determined in the range of 3.5 similar to 70 mu mol/L and the detection limit was 0.6 mu mol/L.