In vivo wound healing activity of gels containing Cecropia pachystachya leaves

ObjectivesTo evaluate the in-vivo wound healing and in-vitro antioxidant activity of gels containing the ethyl acetate extract of Cecropia pachystachya (ECP) 2% and 5%, and to perform the chemical fingerprint of ECP by HPLC-DAD. MethodsThe wound healing activity of the gels was evaluated for 21 days, using the excision model in rats followed by histopathological and histomorphometric analysis. The in-vitro antioxidant effect of ECP was investigated using 1-diphenyl-2-picrylhydrazyl, reducing power, -carotene bleaching and thiobarbituric acid reactive substances assays. Also, a HPLC analysis was performed to identify the chemical markers orientin, iso-orientin and chlorogenic acid. Key findingsThe group of animals treated with ECP 5% presented oedema and inflammatory infiltrate with less intensity than the other groups. Both ECP 2% and 5% gels showed less neovascularization and cellularity, and better tissue repair when compared to the control, which showed a younger and homogeneous tissue. ConclusionsThis study had demonstrated that the ECP gels promoted the acceleration of the healing process when compared to the control group. Wound contraction, angiogenesis, epithelialization and the collagen deposition support further evaluation of C. pachystachya leaves in the topical treatment and management of skin wounds.