Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules

被引：4

作者：

Royo, Felix ^{[1
,2
]}

Azkargorta, Mikel ^{[1
]}

Lavin, Jose L. ^{[1
]}

Clos-Garcia, Marc ^{[1
]}

Cortazar, Ana R. ^{[1
,3
]}

Gonzalez-Lopez, Monika ^{[1
]}

Barcena, Laura ^{[1
]}

del Portillo, Hernando A. ^{[4
,5
,6
]}

Yanez-Mo, Maria ^{[7
]}

Marcilla, Antonio ^{[8
,9
]}

Borras, Francesc E. ^{[10
,11
,12
]}

Peinado, Hector ^{[13
]}

Guerrero, Isabel ^{[14
]}

Vales-Gomez, Mar ^{[15
]}

Cereijo, Unai ^{[16
]}

Sardon, Teresa ^{[16
]}

Aransay, Ana M. ^{[1
,2
]}

Elortza, Felix ^{[1
,2
]}

Falcon-Perez, Juan M. ^{[1
,2
,17
]}

机构：

[1] Bizkaia Technol Pk, Ctr Cooperat Res Biosci, Bizkaia, Spain

[2] Ctr Invest Biomed Red Enfermedades Hepat & Digest, Madrid, Spain

[3] Inst Salud Carlos III, Ctr Invest Biomed Red Canc, Madrid, Spain

[4] Univ Barcelona, ISGlobal, Hosp Clin, Barcelona, Spain

[5] Hlth Sci Res Inst Germans Trias & Pujol, Badalona, Spain

[6] Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain

[7] Univ Autonoma Madrid, Inst Invest Sanit Princesa, Ctr Biol Mol Severo Ochoa, Dept Biol Mol, Madrid, Spain

[8] Univ Valencia, Area Parasitol, Dept Farm & Tecnol Farmaceut & Parasitol, Valencia, Spain

[9] Univ Valencia, Hlth Res Inst Le Fe, Joint Res Unit Endocrinol Nutr & Clin Dietet, Valencia, Spain

[10] Autonomous Univ Barcelona, Dept Cell Biol Physiol & Immunol, Barcelona, Spain

[11] Germans Trias & Pujol Hlth Sci Res Inst IGTP, REMAR IVECAT Grp, Badalona, Spain

[12] Germans Trias & Pujol Univ Hosp, Nephrol Dept, Can Ruti Campus, Badalona, Spain

[13] Spanish Natl Canc Res Ctr, Mol Oncol Program, Microenvironm & Metastasis Lab, Madrid, Spain

[14] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Tissue & Organ Homeostasis, Madrid, Spain

[15] Spanish Natl Res Council, Spanish Natl Ctr Biotechnol, Madrid, Spain

[16] Anax Biotech, Barcelona, Spain

[17] Basque Fdn Sci, Ikerbasque, Bilbao, Spain

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2021年 / 8卷

关键词：

extracellular vesicles (EVs); exosomes; MLP29; fibroblast; cell crosstalk; immune response; ERBIN; ACTIVATION; PATHWAY; INJURY; BETA; PAK2;

D O I：

10.3389/fcell.2020.613583

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.

引用

页数：10

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