Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

被引：10

作者：

Burkhalter, Kristen L. ^{[1
]}

Savage, Harry M. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, 3156 Rampart Rd, Ft Collins, CO 80521 USA

来源：

EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 04期

关键词：

VENEZUELAN EQUINE ENCEPHALITIS; HOLDING CONDITIONS; WEST-NILE; POOLS; RNA;

D O I：

10.3201/eid2304.161772

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We assayed Zika virus-infected mosquitoes stored at room temperature for <= 30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <= 10 days, and virus RNA was detected in samples held for 30 days.

引用

页码：680 / 681

页数：2

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