Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay

被引：11

作者：

Burkhalter, Kristen L. ^{[1
]}

Savage, Harry M. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, 3156 Rampart Rd, Ft Collins, CO 80521 USA

来源：

EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 04期

关键词：

VENEZUELAN EQUINE ENCEPHALITIS; HOLDING CONDITIONS; WEST-NILE; POOLS; RNA;

D O I：

10.3201/eid2304.161772

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We assayed Zika virus-infected mosquitoes stored at room temperature for <= 30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <= 10 days, and virus RNA was detected in samples held for 30 days.

引用

页码：680 / 681

页数：2

共 7 条

[1] EFFECT OF HOLDING CONDITIONS ON THE DETECTION OF CHIKUNGUNYA AND VENEZUELAN EQUINE ENCEPHALITIS VIRUSES IN MOSQUITO POOLS [J].

Andrews, Elizabeth S. ;

Turell, Michael J. .

JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION, 2016, 32 (01) :51-54

[2]

Beaty BJ., 1995, DIAGNOSTIC PROCEDURE, V7th, P189

[3] Duration of infectivity and RNA of Venezuelan equine encephalitis, West Nile, and yellow fever viruses dried on filter paper and maintained at room temperature [J].

Guzman, H ;

Ding, XH ;

Xiao, SY ;

Tesh, RB .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2005, 72 (04) :474-477

[4] Genetic and serologic properties of zika virus associated with an epidemic, Yap State, Micronesia, 2007 [J].

Lanciotti, Robert S. ;

Kosoy, Olga L. ;

Laven, Janeen J. ;

Velez, Jason O. ;

Lambert, Amy J. ;

Johnson, Alison J. ;

Stanfield, Stephanie M. ;

Duffy, Mark R. .

EMERGING INFECTIOUS DISEASES, 2008, 14 (08) :1232-1239

[5] Inactivation and Environmental Stability of Zika Virus [J].

Mueller, Janis A. ;

Harms, Mirja ;

Schubert, Axel ;

Jansen, Stephanie ;

Michel, Detlef ;

Mertens, Thomas ;

Schmidt-Chanasit, Jonas ;

Muench, Jan .

EMERGING INFECTIOUS DISEASES, 2016, 22 (09) :1685-1687

[6] USE OF MOSQUITOS TO DETECT AND PROPAGATE DENGUE VIRUSES [J].

ROSEN, L ;

GUBLER, D .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1974, 23 (06) :1153-1160

[7] Effect of holding conditions on the detection of west nile viral RNA by reverse transcriptase-polymerase chain reaction from mosquito (Diptera: Culicidae) pools [J].

Turell, MJ ;

Spring, AR ;

Miller, MK ;

Cannon, CE .

JOURNAL OF MEDICAL ENTOMOLOGY, 2002, 39 (01) :1-3

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