Reporter-triggered isothermal exponential amplification strategy in ultrasensitive homogeneous label-free electrochemical nucleic acid biosensing

被引：26

作者：

Nie, Ji ^{[1
]}

Zhang, De-Wen ^{[1
]}

Zhang, Fang-Ting ^{[1
]}

Yuan, Fang ^{[1
]}

Zhou, Ying-Lin ^{[1
]}

Zhang, Xin-Xiang ^{[1
]}

机构：

[1] Peking Univ, Coll Chem, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 47期

基金：

中国国家自然科学基金;

关键词：

TARGET RECYCLING STRATEGY; MOLECULAR BEACON; DNA SENSOR;

D O I：

10.1039/c4cc00475b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) of sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct a homogeneous label-free electrochemical sensor for ultrasensitive DNA detection.

引用

页码：6211 / 6213

页数：3

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