Effects of microcystin-LR on the immune dysfunction and ultrastructure of hepatopancreas in giant freshwater prawn Macrobrachium rosenbergii

被引：34
作者：
Zhang, Yingying [1 ]
Shi, Qiang [1 ]
Wei, Wenzhi [1 ]
Xu, Fei [2 ]
Nie, Fubing [2 ]
Yang, Hui [1 ]
机构：
[1] Yangzhou Univ, Coll Anim Sci & Technol, 48 Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China
[2] Lake Gaobaoshaobo Fishery Adm Comm, 732 Middle Yangzijiang Rd, Yangzhou 225009, Jiangsu, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2019年 / 89卷
基金：
中国国家自然科学基金;
关键词：
Microcystin-LR; Macrobrachium rosenbergii; Hepatopancreas; Immune response; Bacteria; ALKALINE-PHOSPHATASE ACTIVITIES; OXIDATIVE STRESS; PATHOLOGICAL-CHANGES; LARGE SHALLOW; IN-VIVO; CYTOSKELETAL DISRUPTION; CYANOBACTERIAL TOXINS; ANTIOXIDANT RESPONSES; LITOPENAEUS-VANNAMEI; TISSUE DISTRIBUTIONS;
D O I：
10.1016/j.fsi.2019.04.039
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Microcystins (MCs), produced by cyanobacteria, can strongly inhibit the activity of protein phosphatase, and exhibit strong hepatotoxicity. Macrobrachium rosenbergii is an important aquaculture economic species. Cyanobacterial blooms occur frequently during the culture of M. rosenbergii. However, the effects of MCs on the M. rosenbergii immune function have not been studied. In the present study, M. rosenbergii were exposed to environment-related concentrations of MC-LR type (0.5 and 5 mu g/L) for 3 weeks. Hepatopancreatic histology was investigated, and antioxidant enzymes activity, acid phosphatase, alkaline phosphatase and lysozyme activity in hepatopancreas were also analyzed. Results showed that MC-LR could damage M. rosenbergii hepatopancreas, induce hepatopancreatic apoptosis and antioxidant dysfunctions. The expression profiles of major immune-related genes after MC-LR exposure were also detected. Some genes with antibacterial functions were suppressed, and the expression of apoptosis-related genes were up-regulated. After MC-LR exposure, the cumulative mortality of M. rosenbergii infected with Vibrio vulnificus and Aeromonas hydrophila were much higher than the control in a time-dose dependent manner. These results indicated the potential negative influence of MC-LR on the immune function of M. rosenbergii.
引用
收藏
页码：586 / 594
页数：9
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