A nonisotopic estrogen receptor-based assay to detect estrogenic compounds

被引：41

作者：

Garrett, SD ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 12期

关键词：

human estrogen receptor; phytoestrogens; estrogens; rapid screening; flavonoids;

D O I：

10.1038/70773

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used the ligand binding domain of the recombinant human estrogen receptor (hER) to develop a nonisotopic assay for detection of estrogenic compounds. The assay is based on competition of the estrogenic ligand with 17 beta-estradiol for binding to the receptor, which leaves 17 beta-estradiol free to bind to an anti-17 beta-estradiol antibody. Unbound anti-17 beta-estradiol antibody then binds to immobilized 17 beta-estradiol-protein conjugate (to which hER is unable to bind for steric reasons), and is detected by an enzyme-labeled anti-rabbit IgG antibody. We used the assay to detect estrogenic compounds (mainly members of the flavonoid group of plant polyphenols) in a variety of commonly consumed plant foods.

引用

页码：1219 / 1222

页数：4

共 25 条

[11] Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta [J].