A molecular probe for the detection of homopurine sequences

被引：31

作者：

Trkulja, Ivan ^{[1
]}

Biner, Sarah M. ^{[1
]}

Langenegger, Simon M. ^{[1
]}

Haener, Robert ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 01期

关键词：

beacon; DNA; excimer; specificity; triple helix;

D O I：

10.1002/cbic.200600378

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The highly selective detection of homopurine target strands with a triplex-forming molecular probe is described. The binding of a clamp-type oligonucleotide containing two terminally attached pyrene molecules to the target sequence is easily monitored through excimer formation. The oligonucleotide probe allows the efficient discrimination of single nucleotide mismatches because of the high mismatch sensitivity of the triplex formation. © 2007 Wiley-VCH Verlag GmbH & Co KGaA.

引用

页码：25 / 27

页数：3

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