GERMINATION AND SUBSEQUENT INACTIVATION OF BACILLUS SUBTILIS SPORES BY PULSED ELECTRIC FIELD TREATMENT

被引：18

作者：

Shin, J. K. ^{[2
]}

Lee, S. J. ^{[3
]}

Cho, H. Y. ^{[4
]}

Pyun, Y. R. ^{[3
]}

Lee, J. H. ^{[1
]}

Chung, M. S. ^{[1
]}

机构：

[1] Ewha Womans Univ, Dept Food Sci & Technol, Seoul 120750, South Korea

[2] Jeonju Univ, Dept Korean Tradit Food Culture, Jeonju 560759, South Korea

[3] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[4] CHO Korea, Puchon 421741, South Korea

来源：

JOURNAL OF FOOD PROCESSING AND PRESERVATION | 2010年 / 34卷 / 01期

关键词：

BACTERIAL-SPORES; SACCHAROMYCES-CEREVISIAE; TEMPERATURE; OUTGROWTH; MICROORGANISMS; INHIBITION; FOODS;

D O I：

10.1111/j.1745-4549.2008.00321.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The use of high-intensity pulsed electric field (PEF) as a nonthermal method for triggering spore germination was investigated. Spores suspended in 0.01% NaCl solution (ca. 10(8) cells/mL) were treated with an exponential-decay pulse (20-50 kV/cm) at selected temperatures. The inactivation resulting from the direct effect of PEF treatment was less than 0.5 log cycles, even with a relatively high electric field intensity of 40 kV/cm. Most of the surviving PEF-treated spores germinated after inoculation into selected germination media (0.85% NaCl solution, nutrient broth [NB], or tryptic soy broth [TSB]) and subsequent incubation at 37C for 40 min. The extent of germination increased with the treatment temperature and electric field intensity. More than 98% of surviving spores in TSB germinated during incubation after PEF treatment with an electric field strength of 40 kV/cm for 1,000 mu s at 50C. The rate of germination in nutrient-rich TSB medium was higher than that in NB.

引用

页码：43 / 54

页数：12

共 50 条

[31] Effects of pulsed near infrared light (NIR) on Bacillus subtilis spores [J].

Dikec, J. ;

Bechoua, N. ;

Winckler, P. ;

Perrier-Cornet, J. M. .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2022, 234

[32] On the inactivation of Bacillus subtilis spores by surface streamer discharge in humid air caused by reactive species [J].

Dolezalova, Eva ;

Prukner, Vaclav ;

Kuzminova, Anna ;

Simek, Milan .

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 2020, 53 (24)

[33] Modelling the effect of sub(lethal) heat treatment of Bacillus subtilis spores on germination rate and outgrowth to exponentially growing vegetative cells [J].

Smelt, J. P. P. M. ;

Bos, A. P. ;

Kort, R. ;

Brul, S. .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 128 (01) :34-40

[34] Sporulation at reduced water activity impairs germination kinetics of Bacillus subtilis spores [J].

Freire, Victor ;

Condon, Santiago ;

Gayan, Elisa .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2025, 91 (07)

[35] Inactivation effects of combined thermosonication and potassium sorbate treatments on Bacillus subtilis spores [J].

Li, Jiajia ;

Zhang, Zhong ;

Li, Qinghuan ;

Liu, Yongxia ;

Liu, Yichang .

FOOD SCIENCE AND BIOTECHNOLOGY, 2024, 33 (14) :3357-3366

[36] Effects of combining lysozyme, ultrasound, and heat treatments on the inactivation of Bacillus subtilis spores [J].

Tian, Peng ;

Wang, Xujian ;

Zhu, Gaili ;

Zhang, Haojie ;

Zhang, Zhong .

ARCHIVES OF MICROBIOLOGY, 2025, 207 (08)

[37] Pulsed Electric Field Treatment of Microalgae: Inactivation Tendencies and Energy Consumption [J].

Qin, Si ;

Timoshkin, Igor V. ;

Maclean, Michelle ;

Wilson, Mark P. ;

MacGregor, Scott J. ;

Given, Martin J. ;

Anderson, John G. ;

Wang, Tao .

IEEE TRANSACTIONS ON PLASMA SCIENCE, 2014, 42 (10) :3191-3196

[38] Pulsed light inactivation of Bacillus subtilis vegetative cells in suspensions and spices [J].

Nicorescu, I. ;

Nguyen, B. ;

Moreau-Ferret, M. ;

Agoulon, A. ;

Chevalier, S. ;

Orange, N. .

FOOD CONTROL, 2013, 31 (01) :151-157

[39] Uptake of UVc induced photoproducts of dipicolinic acid by Bacillus subtilis spores - Effects on the germination and UVc resistance of the spores [J].

Dikec, J. ;

Pacheco, M. ;

Lavaud, M. ;

Winckler, P. ;

Perrier-Cornet, J. M. .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2022, 236

[40] Germination and inactivation of Bacillus coagulans and Alicyclobacillus acidoterrestris spores by high hydrostatic pressure treatment in buffer and tomato sauce [J].

Vercammen, Anne ;

Vivijs, Bram ;

Lurquin, Ine ;

Michiels, Chris W. .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 152 (03) :162-167

← 1 2 3 4 5 →