GERMINATION AND SUBSEQUENT INACTIVATION OF BACILLUS SUBTILIS SPORES BY PULSED ELECTRIC FIELD TREATMENT

被引：18
作者：
Shin, J. K. [2 ]
Lee, S. J. [3 ]
Cho, H. Y. [4 ]
Pyun, Y. R. [3 ]
Lee, J. H. [1 ]
Chung, M. S. [1 ]
机构：
[1] Ewha Womans Univ, Dept Food Sci & Technol, Seoul 120750, South Korea
[2] Jeonju Univ, Dept Korean Tradit Food Culture, Jeonju 560759, South Korea
[3] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea
[4] CHO Korea, Puchon 421741, South Korea
来源：
JOURNAL OF FOOD PROCESSING AND PRESERVATION | 2010年 / 34卷 / 01期
关键词：
BACTERIAL-SPORES; SACCHAROMYCES-CEREVISIAE; TEMPERATURE; OUTGROWTH; MICROORGANISMS; INHIBITION; FOODS;
D O I：
10.1111/j.1745-4549.2008.00321.x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The use of high-intensity pulsed electric field (PEF) as a nonthermal method for triggering spore germination was investigated. Spores suspended in 0.01% NaCl solution (ca. 10(8) cells/mL) were treated with an exponential-decay pulse (20-50 kV/cm) at selected temperatures. The inactivation resulting from the direct effect of PEF treatment was less than 0.5 log cycles, even with a relatively high electric field intensity of 40 kV/cm. Most of the surviving PEF-treated spores germinated after inoculation into selected germination media (0.85% NaCl solution, nutrient broth [NB], or tryptic soy broth [TSB]) and subsequent incubation at 37C for 40 min. The extent of germination increased with the treatment temperature and electric field intensity. More than 98% of surviving spores in TSB germinated during incubation after PEF treatment with an electric field strength of 40 kV/cm for 1,000 mu s at 50C. The rate of germination in nutrient-rich TSB medium was higher than that in NB.
引用
收藏
页码：43 / 54
页数：12
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