Metabolism of acenaphthylene via 1,2-dihydroxynaphthalene and catechol by Stenotrophomonas sp RMSK

被引：24

作者：

Nayak, Anand S. ^{[1
]}

Veeranagouda, Yaligara ^{[2
]}

Lee, Kyoung ^{[2
]}

Karegoudar, T. B. ^{[1
]}

机构：

[1] Gulbarga Univ, Dept Biochem, Gulbarga 585106, Karnataka, India

[2] Changwon Natl Univ, Dept Microbiol, Changwon Si 641773, Kyongnam, South Korea

来源：

BIODEGRADATION | 2009年 / 20卷 / 06期

关键词：

Acenaphthylene; Stenotrophomonas; 1,2-Dihydroxynaphthalene; Biodegradation; Catechol-1,2-dioxygenase; AROMATIC-HYDROCARBONS ACENAPHTHENE; PSEUDOMONAS-PUTIDA; PATHWAY; PURIFICATION;

D O I：

10.1007/s10532-009-9271-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Stenotrophomonas sp. RMSK capable of degrading acenaphthylene as a sole source of carbon and energy was isolated from coal sample. Metabolites produced were analyzed and characterized by TLC, HPLC and mass spectrometry. Identification of naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid, 1,2-dihydroxynaphthalene, salicylate and detection of key enzymes namely 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase in the cell free extract suggest that acenaphthylene metabolized via 1,2-dihydroxynaphthalene, salicylate and catechol. The terminal metabolite, catechol was then metabolized by catechol-1,2-dioxygenase to cis,cis-muconic acid, ultimately forming TCA cycle intermediates. Based on these studies, the proposed metabolic pathway in strain RMSK is, acenaphthylene -> naphthalene-1,8-dicarboxylic acid -> 1-naphthoic acid -> 1,2-dihydroxynaphthalene -> salicylic acid -> catechol -> cis,cis-muconic acid.

引用

页码：837 / 843

页数：7

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