In vivo monitoring of rat brain endocannabinoids using solid-phase microextraction

被引：10

作者：

Aslam, Momna ^{[1
]}

Feleder, Carlos ^{[2
]}

Newsom, Ryan J. ^{[3
]}

Campeau, Serge ^{[3
]}

Musteata, Florin Marcel ^{[1
]}

机构：

[1] Albany Coll Pharm & Hlth Sci, Dept Pharmaceut Sci, 106 New Scotland Ave, Albany, NY 12208 USA

[2] Univ Ciencias Empresariales & Sociales, Inst Salud Publ & Efectividad Clin, Dept Invest, C1061ABA CABA, Buenos Aires, DF, Argentina

[3] Univ Colorado, Dept Psychol & Neurosci, 345 UCB, Boulder, CO 80309 USA

来源：

BIOANALYSIS | 2019年 / 11卷 / 16期

关键词：

anandamide; 2-arachidonoyl glycerol; brain; endocannabinoids; in vivo analysis; solid-phase microextraction; LC-MS/MS METHOD; CEREBROSPINAL-FLUID; MS METHOD; ANANDAMIDE; QUANTIFICATION; STRESS; ACID; CB1;

D O I：

10.4155/bio-2019-0144

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: Solid-phase microextraction is proposed to measure concentrations of anandamide and 2arachidonoyl glycerol in live rat brains in response to stress. Materials & methods: Solid-phase microextraction fibers were prepared from steel with 1.5 mm extraction coating. 24 male rats were divided into groups based on brain region, stria terminalis or posterior hypothalamus and loud noise or control groups. The fibers were desorbed in acetonitrile-water (75:25) and analyzed by ultraperformance LC-MS/MS. The linear range of the method was 0.05-50 ng/ml and the in vivo concentrations were found to be between 0.3 and 40 ng/ml. Conclusion: The new approach was successfully used to determine the concentrations of anandamide and 2-arachidonoyl glycerol in vivo and could be used in the future to measure other endogenous compounds.

引用

页码：1523 / 1534

页数：12

共 48 条

[1]

Ashton JC, 2017, ENDOCANNABINOID SYSTEM: GENETICS, BIOCHEMISTRY, BRAIN DISORDERS, AND THERAPY, P115, DOI 10.1016/B978-0-12-809666-6.00005-8

[2] CB1 receptor-mediated control of the release of endocannabinoids (as assessed by microdialysis coupled with LC/MS) in the rat hypothalamus [J].

Bequet, Frederic ;

Uzabiaga, Francoise ;

Desbazeille, Mathieu ;

Ludwiczak, Pascal ;

Maftouh, Mohamed ;

Picard, Claudine ;

Scatton, Bernard ;

Le Fur, Gerard .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2007, 26 (12) :3458-3464

[3] Solid phase microextraction fills the gap in tissue sampling protocols [J].

Bojko, Barbara ;

Gorynski, Krzysztof ;

Gomez-Rios, German Augusto ;

Knaak, Jan Matthias ;

Machuca, Tiago ;

Spetzler, Vinzent Nikolaus ;

Cudjoe, Erasmus ;

Hsin, Michael ;

Cypel, Marcelo ;

Selzner, Markus ;

Liu, Mingyao ;

Keshavjee, Shaf ;

Pawliszyn, Janusz .

ANALYTICA CHIMICA ACTA, 2013, 803 :75-81

[4] Quantification of brain endocannabinoid levels: methods, interpretations and pitfalls [J].

Buczynski, Matthew W. ;

Parsons, Loren H. .

BRITISH JOURNAL OF PHARMACOLOGY, 2010, 160 (03) :423-442

[5] Troubleshooting in LC-MS/MS method for determining endocannabinoid and endocannabinoid-like molecules in rat brain structures applied to assessing the brain endocannabinoid/endovanilloid system significance [J].

Bystrowska, Beata ;

Smaga, Irena ;

Tyszka-Czochara, Malgorzata ;

Filip, Malgorzata .

TOXICOLOGY MECHANISMS AND METHODS, 2014, 24 (04) :315-322

[6] Solid-Phase Microextraction: A Complementary In Vivo Sampling Method to Microdialysis [J].

Cudjoe, Erasmus ;

Bojko, Barbara ;

de Lannoy, Ines ;

Saldivia, Victor ;

Pawliszyn, Janusz .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (46) :12124-12126

[7]

Cudjoe E, 2012, BIOANALYSIS, V4, P2605, DOI [10.4155/BIO.12.250, 10.4155/bio.12.250]

[8] Reversible inactivation of the auditory thalamus disrupts HPA axis habituation to repeated loud noise stress exposures [J].

Day, Heidi E. W. ;

Masini, Cher V. ;

Campeau, Serge .

BRAIN RESEARCH, 2009, 1276 :123-130

[9] Abnormalities in the cerebrospinal fluid levels of endocannabinoids in multiple sclerosis [J].

Di Filippo, M. ;

Pini, L. A. ;

Pelliccioli, G. P. ;

Calabresi, P. ;

Sarchielli, P. .

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2008, 79 (11) :1224-1229

[10] A quantitiative LC-MS/MS method for the measurement of arachidonic acid, prostanoids, endocannabinoids, N-acylethanolamines and steroids in human plasma [J].

Gachet, Maria Salome ;

Rhyn, Peter ;

Bosch, Oliver G. ;

Quednow, Boris B. ;

Gertsch, Jtirg .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2015, 976 :6-18

← 1 2 3 4 5 →