Site-selective reactions of imperfectly matched DNA with small chemical molecules: applications in mutation detection

被引：14

作者：

Bui, CT ^{[1
]}

Rees, K ^{[1
]}

Lambrinakos, A ^{[1
]}

Bedir, A ^{[1
]}

Cotton, RGH ^{[1
]}

机构：

[1] Univ Melbourne, Genom Disorders Res Ctr, Fitzroy, Vic 3065, Australia

来源：

BIOORGANIC CHEMISTRY | 2002年 / 30卷 / 04期

关键词：

imperfectly matched DNA; mismatched DNA; unmatched DNA; abasic DNA; site-selective reactions of DNA; detection of mutations;

D O I：

10.1016/S0045-2068(02)00019-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The last decade has witnessed many exciting scientific publications associated with site-selective reactions of small chemical molecules with imperfectly matched DNA. Typical examples are carbodiimide, hydroxylamine, potassium permanganate, osmium tetroxide, chemical tagging probes, biotinylated, chemiluminescent and fluorescent probes, and all of them selectively react with imperfectly matched DNA. More recently, some therapeutic agents including DNA intercalating drugs and groove binders have been found to promote the in vivo repair system to recognize and repair the mismatch more effectively. The results have established a novel method for detection of mismatches. Development of new chemical reactions for detection of imperfectly matched DNA and mutations is a rapidly growing field and has attracted significant interest of scientists from both chemical and biological fields and it is the main focus of this review. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：216 / 232

页数：17

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