Discovery of a size-record breaking green-emissive fluorophore: small, smaller, HINA

被引：15

作者：

Kang, Rui ^{[1
]}

Talamini, Laura ^{[2
]}

D'Este, Elisa ^{[3
]}

Estevao, Bianca Martins ^{[2
]}

De Cola, Luisa ^{[1
,2
]}

Klopper, Wim ^{[1
,4
]}

Biedermann, Frank ^{[1
]}

机构：

[1] Karlsruhe Inst Technol KIT, Inst Nanotechnol INT, Hermann Von Helmholtz Pl 1, D-76344 Eggenstein Leapaldshafen, Germany

[2] Univ Strasbourg, Inst Sci & Ingn Supramol ISIS, CNRS, 8Rue Gaspard Mange, F-67083 Strasbourg, France

[3] Max Plank Inst Med Res, Opt Microscopy Facil, Jahnstr 29, D-69120 Heidelberg, Germany

[4] Karlsruhe Inst Technol KIT, Inst Phys Chem IPC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany

来源：

CHEMICAL SCIENCE | 2021年 / 12卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

53;

D O I：

10.1039/d0sc05557c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Astonishingly, 3-hydroxyisonicotinealdehyde (HINA) is despite its small size a green-emitting push-pull fluorophore in water (QY of 15%) and shows ratiometric emission response to biological relevant pH differences (pK(a2) similar to 7.1). Moreover, HINA is the first small-molecule fluorophore reported that possesses three distinctly emissive protonation states. This fluorophore can be used in combination with metal complexes for fluorescent-based cysteine detection in aqueous media, and is readily taken up by cells. The theoretical description of HINA's photophysics remains challenging, even when computing Franck-Condon profiles via coupled-cluster calculations, making HINA an interesting model for future method development.

引用

页码：1392 / 1397

页数：6

共 53 条

[11] A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging [J].