Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19

被引：39

作者：

Goh, Yun Shan ^{[1
,2
]}

Chavatte, Jean-Marc ^{[3
]}

Jieling, Alicia Lim ^{[3
]}

Lee, Bernett ^{[2
]}

Hor, Pei Xiang ^{[1
,2
]}

Amrun, Siti Naqiah ^{[1
,2
]}

Lee, Cheryl Yi-Pin ^{[1
,2
]}

Chee, Rhonda Sin-Ling ^{[1
,2
]}

Wang, Bei ^{[2
]}

Lee, Chia Yin ^{[2
]}

Ngoh, Eve Zhi Xian ^{[2
]}

Wang, Cheng-, I ^{[2
]}

Young, Barnaby Edward ^{[3
,4
,5
]}

Tambyah, Paul A. ^{[3
,6
]}

Kalimuddin, Shirin ^{[7
,8
]}

Pada, Surinder ^{[9
]}

Tan, Seow-Yen ^{[10
]}

Sun, Louisa Jin ^{[11
]}

Chen, Mark I-Cheng ^{[3
,12
,13
]}

Leo, Yee-Sin ^{[3
,4
,5
,14
,15
]}

Lye, David C. ^{[3
,4
,5
,14
,15
]}

Ng, Lisa F. P. ^{[1
,2
,16
,17
,18
,19
]}

Lin, Raymond Tzer Pin ^{[3
,14
,15
]}

Renia, Laurent ^{[1
,2
,19
]}

机构：

[1] ASTAR, Infect Dis Labs ID Labs, Immunos, Biopolis, Singapore 138648, Singapore

[2] ASTAR, Singapore Immunol Network, Immunos, Biopolis, Singapore 138648, Singapore

[3] Natl Ctr Infect Dis, 16 Jalan Tan Tock Seng, Singapore 308442, Singapore

[4] Tan Tock Seng Hosp, Dept Infect Dis, 11 Jalan Tan Tock Seng, Singapore 308433, Singapore

[5] Nanyang Technol Univ, Lee Kong Chian Sch Med, 11 Mandalay Rd, Singapore 308232, Singapore

[6] Natl Univ Singapore Hosp, Dept Med, 5 Lower Kent Ridge Rd, Singapore 119074, Singapore

[7] Singapore Gen Hosp, Dept Infect Dis, 31 Third Hosp Ave, Singapore 168753, Singapore

[8] Duke NUS Med Sch, Emerging Infect Dis Program, 8 Coll Rd, Singapore 169857, Singapore

[9] Ng Teng Fong Hosp, Div Infect Dis, 1 Jurong East St 21, Singapore 609606, Singapore

[10] Changi Gen Hosp, Dept Infect Dis, 2 Simei St 3, Singapore 529889, Singapore

[11] Alexandra Hosp, 378 Alexandra Rd, Singapore 159964, Singapore

[12] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, 12 Sci Dr 2, Singapore 117549, Singapore

[13] Natl Univ Hlth Syst, 12 Sci Dr 2, Singapore 117549, Singapore

[14] Natl Univ Singapore, Yong Loo Lin Sch Med, 10 Med Dr, Singapore 117597, Singapore

[15] Natl Univ Hlth Syst, 10 Med Dr, Singapore 117597, Singapore

[16] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore 117596, Singapore

[17] Univ Liverpool, Natl Inst Hlth Res, Hlth Protect Res Unit Emerging & Zoonot Infect, Liverpool, Merseyside, England

[18] Univ Liverpool, Inst Infect Vet & Ecol Sci, Liverpool, Merseyside, England

[19] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Microbiol & Immunol, Singapore 117597, Singapore

来源：

CELL REPORTS MEDICINE | 2021年 / 2卷 / 02期

基金：

英国医学研究理事会;

关键词：

VACCINE; SARS;

D O I：

10.1016/j.xcrm.2021.100193

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Early detection of infection is crucial to limit the spread of coronavirus disease 2019 (COVID-19). Here we develop a flow cytometry-based assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein antibodies in individuals with COVID-19. The assay detects specific immunoglobulin M (IgM), IgA, and IgG in individuals with COVID-19 and also acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of infection. Furthermore, asymptomatic individuals with COVID-19 also develop specific IgM, IgA, and IgG, with IgG1 being the most dominant subclass. Although the antibody levels are lower in asymptomatic infection, the assay is highly sensitive and detects 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic and asymptomatic infections, which may otherwise remain undetected.

引用

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