Single-cell transcriptomics reveals specific RNA editing signatures in the human brain

被引：55

作者：

Picardi, Ernesto ^{[1
,2
]}

Horner, David S. ^{[3
]}

Pesole, Graziano ^{[1
,2
]}

机构：

[1] Univ Bari, Dept Biosci Biotechnol & Biopharmaceut, I-70126 Bari, Italy

[2] CNR, Inst Biomembranes & Bioenerget, I-70126 Bad, Italy

[3] Univ Milan, Dept Biosci, I-20133 Milan, Italy

来源：

RNA | 2017年 / 23卷 / 06期

关键词：

RNA editing; single cell; RNA-seq; transcriptome; DNA; CONTRIBUTOR; DIVERSITY; TISSUES;

D O I：

10.1261/rna.058271.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

While RNA editing by A-to-I deamination is a requisite for neuronal function in humans, it is under-investigated in single cells. Here we fill this gap by analyzing RNA editing profiles of single cells from the brain cortex of living human subjects. We show that RNA editing levels per cell are bimodally distributed and distinguish between major brain cell types, thus providing new insights into neuronal dynamics.

引用

页码：860 / 865

页数：6

共 50 条

[1] Transcriptomics and single-cell RNA-sequencing
Chambers, Daniel C.
Carew, Alan M.
Lukowski, Samuel W.
Powell, Joseph E.
RESPIROLOGY, 2019, 24 (01) : 29 - 36
[2] Single-cell transcriptomics reveals distinct inflammation-induced microglia signatures
Sousa, Carole
Golebiewska, Anna
Poovathingal, Suresh K.
Kaoma, Tony
Pires-Afonso, Yolanda
Martina, Silvia
Coowar, Djalil
Azuaje, Francisco
Skupin, Alexander
Balling, Rudi
Biber, Knut
Niclou, Simone P.
Michelucci, Alessandro
EMBO REPORTS, 2018, 19 (11)
[3] Transcriptomics of Human Brain Tissue in Parkinson's Disease: a Comparison of Bulk and Single-cell RNA Sequencing
Fiorini, Michael R.
Dilliott, Allison A.
Thomas, Rhalena A.
Farhan, Sali M. K.
MOLECULAR NEUROBIOLOGY, 2024, 61 (11) : 8996 - 9015
[4] Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Smits, Lisa M.
Magni, Stefano
Kinugawa, Kaoru
Grzyb, Kamil
Luginbuhl, Joachim
Sabate-Soler, Sonia
Bolognin, Silvia
Shin, Jay W.
Mori, Eiichiro
Skupin, Alexander
Schwamborn, Jens C.
CELL AND TISSUE RESEARCH, 2020, 382 (03) : 463 - 476
[5] Elucidating memory in the brain via single-cell transcriptomics
Sullivan, Kaitlin E.
Kendrick, Rennie M.
Cembrowski, Mark S.
JOURNAL OF NEUROCHEMISTRY, 2021, 157 (04) : 982 - 992
[6] Single-cell transcriptomics as a framework and roadmap for understanding the brain
Cembrowski, Mark S.
JOURNAL OF NEUROSCIENCE METHODS, 2019, 326
[7] Single-cell transcriptomics reveals variable trajectories of CSPCs in the progression of osteoarthritis
Qi, Lingbin
Wang, Jian
Chen, Xian
Ding, Yanhui
Ling, Bin
Wang, Wenjun
Xu, Jun
Xue, Zhigang
HELIYON, 2022, 8 (11)
[8] Single-cell transcriptomics reveals immune infiltrate in sepsis
Tu, Xusheng
Huang, He
Xu, Shilei
Li, Caifei
Luo, Shaoning
FRONTIERS IN PHARMACOLOGY, 2023, 14
[9] Human organomics: a fresh approach to understanding human development using single-cell transcriptomics
Camp, J. Gray
Treutlein, Barbara
DEVELOPMENT, 2017, 144 (09): : 1584 - 1587
[10] Single-cell RNA sequencing of human prostate basal epithelial cells reveals zone-specific cellular populations and gene expression signatures
Vellky, Jordan E.
Wu, Yaqi
Moline, Daniel
Drnevich, Jenny
Maienschein-Cline, Mark
Valyi-Nagy, Klara
Kajdacsy-Balla, Andre
Vander Griend, Donald J.
JOURNAL OF PATHOLOGY, 2024, 262 (02) : 212 - 225

← 1 2 3 4 5 →