Pgp3 Antibody Enzyme-Linked Immunosorbent Assay, a Sensitive and Specific Assay for Seroepidemiological Analysis of Chlamydia trachomatis Infection

被引:68
作者
Wills, Gillian S. [1 ]
Horner, Patrick J. [5 ]
Reynolds, Rosy [6 ]
Johnson, Anne M. [2 ]
Muir, David A. [3 ]
Brown, David W. [4 ]
Winston, Alan [1 ]
Broadbent, Andrew J. [1 ]
Parker, David [7 ]
McClure, Myra O. [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Wright Fleming Inst, Jefferiss Trust Labs, London W2 1PG, England
[2] Univ Coll London, Res Dept Infect & Populat Hlth, Colindale, England
[3] Imperial Coll Healthcare NHS Trust, St Marys Hosp, Dept Diagnost Virol, Colindale, England
[4] Hlth Protect Agcy, Ctr Infect, Colindale, England
[5] Univ Bristol, Dept Social Med, Bristol, Avon, England
[6] N Bristol NHS Trust, Dept Med Microbiol, Bristol, Avon, England
[7] Novel Consulting, Crown House, Home Gardens, Dartford, England
基金
英国医学研究理事会;
关键词
INCLUSION-FORMING UNITS; PLASMID PROTEIN PGP3; HEAT-SHOCK-PROTEIN; PNEUMONIAE INFECTION; NUCLEOTIDE-SEQUENCE; ECTOPIC PREGNANCY; DIAGNOSTIC-VALUE; OUTER-MEMBRANE; COMMON PLASMID; C-TRACHOMATIS;
D O I
10.1128/CVI.00021-09
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Understanding of the burden of Chlamydia trachomatis infection and its clinical sequelae is hampered by the absence of accurate, well-characterized tests using serological methods to determine past exposure to infection. An "in-house" immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) based on the C. trachomatis-specific antigen Pgp3 was produced and evaluated against three commercial ELISAs derived from the major outer membrane protein: the Medac pELISA plus, the Savyon SeroCT-IgG ELISA, and the Ani Labsystems IgG enzyme immunoassay. Sensitivities and specificities were determined using sera from both male and female patients (n = 356) for whom C. trachomatis had been detected in the lower genital tract at least 1 month prior to the testing of the sample and from 722 Chlamydia-negative children aged 2 to 13 years. The Pgp3 ELISA was significantly more sensitive (57.9% [95% confidence interval {95% CI}, 52.7 to 62.9%]) than the Ani Labsystems (49.2% [95% CI, 44.0 to 54.3%]; P = 0.003), SeroCT (47.2% [95% CI, 42.1 to 52.4%]; P < 0.0005), and Medac (44.4% [95% CI, 39.3 to 49.6%]; P < 0.0005) ELISAs. The Pgp3, Ani Labsystems, and SeroCT assays, but not the Medac assay, had significantly higher sensitivity for female specimens than for male specimens (73.8 versus 44.2%, 59.8 versus 40.5%, 55.5 versus 40%, and 45.7 versus 43.7%, respectively). For female patients, the Pgp3 assay was 14.0% (95% CI, 5.5 to 22.5%) more sensitive than the next most sensitive ELISA, the Ani Labsystems assay (P = 0.001). There was no significant difference in specificity between the Pgp3 (97.6% [95% CI, 96.2 to 98.6%]), Ani Labsystems (99% [95% CI, 97.7 to 99.6%]), SeroCT (97.2% [95% CI, 95.7 to 98.2%]), and Medac (96% [95% CI, 94.3 to 97.2%]) ELISAs. None of the ELISAs showed evidence of cross-reactivity with antibodies to Chlamydia pneumoniae.
引用
收藏
页码:835 / 843
页数:9
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