MChip: A tool for influenza surveillance

被引：62

作者：

Dawson, Erica D.

Moore, Chad L.

Smagala, James A.

Dankbar, Daniela M.

Mehlmann, Martin

Townsend, Michael B.

Smith, Catherine B.

Cox, Nancy J.

Kuchta, Robert D.

Rowlen, Kathy L.

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[2] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA 30333 USA

[3] InDevR LLC, Boulder, CO 80301 USA

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 22期

关键词：

D O I：

10.1021/ac061739f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The design and characterization of a low-density microarray for subtyping influenza A is presented. The microarray consisted of 15 distinct oligonucleotides designed to target only the matrix gene segment of influenza A. An artificial neural network was utilized to automate microarray image interpretation. The neural network was trained to recognize fluorescence image patterns for 68 known influenza viruses and subsequently used to identify 53 unknowns in a blind study that included 39 human patient samples and 14 negative control samples. The assay exhibited a clinical sensitivity of 95% and clinical specificity of 92%.

引用

收藏

页码：7610 / 7615

页数：6

相关论文

共 43 条

[21] Optimization of fragmentation conditions for microarray analysis of viral RNA [J].

Mehlmann, M ;

Townsend, MB ;

Stears, RL ;

Kuchta, RD ;

Rowlen, KL .

ANALYTICAL BIOCHEMISTRY, 2005, 347 (02) :316-323

[22] Robust sequence selection method used to develop the FluChip diagnostic microarray for influenza virus [J].

Mehlmann, Martin ;

Dawson, Erica D. ;

Townsend, Michael B. ;

Smagala, James A. ;

Moore, Chad L. ;

Smith, Catherine B. ;

Cox, Nancy J. ;

Kuchta, Robert D. ;

Rowlen, Kathy L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2857-2862

[23] Assembly and budding of influenza virus [J].

Nayak, DP ;

Hui, EKW ;

Barman, S .

VIRUS RESEARCH, 2004, 106 (02) :147-165

[24] Influenza A H5N1 detection [J].

Ng, EKO ;

Cheng, PKC ;

Ng, AYY ;

Hoang, TL ;

Lim, WWL .

EMERGING INFECTIOUS DISEASES, 2005, 11 (08) :1303-1305

[25] Specific detection of H5N1 avian influenza A virus in field specimens by a one-step RT-PCR assay [J].

Ng, LFP ;

Barr, I ;

Nguyen, T ;

Noor, SM ;

Tan, RSP ;

Agathe, LV ;

Gupta, S ;

Khalil, H ;

To, TL ;

Hassan, SS ;

Ren, EC .

BMC INFECTIOUS DISEASES, 2006, 6 (1)

[26] Large-scale sequence analysis of avian influenza isolates [J].

Obenauer, JC ;

Denson, J ;

Mehta, PK ;

Su, XP ;

Mukatira, S ;

Finkelstein, DB ;

Xu, XQ ;

Wang, JH ;

Ma, J ;

Fan, YP ;

Rakestraw, KM ;

Webster, RG ;

Hoffmann, E ;

Krauss, S ;

Zheng, J ;

Zhang, ZW ;

Naeve, CW .

SCIENCE, 2006, 311 (5767) :1576-1580

[27]

Park KY, 1997, ACTA VIROL, V41, P231

[28] Single-step multiplex reverse transcription-polymerase chain reaction (RT-PCR) for influenza A virus subtype H5N1 detection [J].

Payungporn, S ;

Phakdeewirot, P ;

Chutinimitkul, S ;

Theamboonlers, A ;

Keawcharoen, J ;

Oraveerakul, K ;

Amonsin, A ;

Poovorawan, Y .

VIRAL IMMUNOLOGY, 2004, 17 (04) :588-593

[29] Single step multiplex real-time RT-PCR for H5N1 influenza A virus detection [J].

Payungporn, S ;

Chutinimitkul, S ;

Chaisingh, A ;

Damrongwantanapokin, S ;

Buranathai, C ;

Amonsin, A ;

Theamboonlers, A ;

Poovorawan, Y .

JOURNAL OF VIROLOGICAL METHODS, 2006, 131 (02) :143-147

[30] Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription-polymerase chain reaction (RT-PCR) and agarose gel electrophoresis [J].

Poddar, SK .

JOURNAL OF VIROLOGICAL METHODS, 2002, 99 (1-2) :63-70

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