MChip: A tool for influenza surveillance

被引：62

作者：

Dawson, Erica D.

Moore, Chad L.

Smagala, James A.

Dankbar, Daniela M.

Mehlmann, Martin

Townsend, Michael B.

Smith, Catherine B.

Cox, Nancy J.

Kuchta, Robert D.

Rowlen, Kathy L.

机构：

[1] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA

[2] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA 30333 USA

[3] InDevR LLC, Boulder, CO 80301 USA

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 22期

关键词：

D O I：

10.1021/ac061739f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The design and characterization of a low-density microarray for subtyping influenza A is presented. The microarray consisted of 15 distinct oligonucleotides designed to target only the matrix gene segment of influenza A. An artificial neural network was utilized to automate microarray image interpretation. The neural network was trained to recognize fluorescence image patterns for 68 known influenza viruses and subsequently used to identify 53 unknowns in a blind study that included 39 human patient samples and 14 negative control samples. The assay exhibited a clinical sensitivity of 95% and clinical specificity of 92%.

引用

页码：7610 / 7615

页数：6

共 43 条

[1]

Blalock E. M., 2003, A beginner's guide to microarrays

[2] Diagnostic markers of ovarian cancer by high-throughput antigen cloning and detection on arrays [J].

Chatterjee, M ;

Mohapatra, S ;

Ionan, A ;

Bawa, G ;

Ali-Fehmi, R ;

Wang, XJ ;

Nowak, J ;

Ye, B ;

Nahhas, FA ;

Lu, K ;

Witkin, SS ;

Fishman, D ;

Munkarah, A ;

Morris, R ;

Levin, NK ;

Shirley, NN ;

Tromp, G ;

Abrams, J ;

Draghici, S ;

Tainsky, MA .

CANCER RESEARCH, 2006, 66 (02) :1181-1190

[3] A novel influenza A virus mitochondrial protein that induces cell death [J].

Chen, WS ;

Calvo, PA ;

Malide, D ;

Gibbs, J ;

Schubert, U ;

Bacik, I ;

Basta, S ;

O'Neill, R ;

Schickli, J ;

Palese, P ;

Henklein, P ;

Bennink, JR ;

Yewdell, JW .

NATURE MEDICINE, 2001, 7 (12) :1306-1312

[4] Go with the microflow [J].

Clayton, J .

NATURE METHODS, 2005, 2 (08) :621-627

[5]

DAWSON ED, UNPUB ANAL CHEM

[6]

*DEP HLTH HUM SERV, 2006, HHS PAND INFL PLAN

[7] A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control [J].

Di Trani, Livia ;

Bedini, Barbara ;

Donatelli, Isabella ;

Campitelli, Laura ;

Chiappini, Barbara ;

De Marco, Maria Alessandra ;

Delogu, Mauro ;

Buonavoglia, Canio ;

Vaccari, Gabriele .

BMC INFECTIOUS DISEASES, 2006, 6 (1)

[8] Detection of influenza A viruses from different species by PCR amplification of conserved sequences in the matrix gene [J].

Fouchier, RAM ;

Bestebroer, TM ;

Herfst, S ;

Van der Kemp, L ;

Rimmelzwaan, GF ;

Osterhaus, ADME .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (11) :4096-4101

[9] Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution [J].

Ghedin, E ;

Sengamalay, NA ;

Shumway, M ;

Zaborsky, J ;

Feldblyum, T ;

Subbu, V ;

Spiro, DJ ;

Sitz, J ;

Koo, H ;

Bolotov, P ;

Dernovoy, D ;

Tatusova, T ;

Bao, YM ;

St George, K ;

Taylor, J ;

Lipman, DJ ;

Fraser, CM ;

Taubenberger, JK ;

Salzberg, SL .

NATURE, 2005, 437 (7062) :1162-1166

[10] EVOLUTIONARY ANALYSIS OF THE INFLUENZA-A VIRUS M-GENE WITH COMPARISON OF THE M1 AND M2 PROTEINS [J].

ITO, T ;

GORMAN, OT ;

KAWAOKA, Y ;

BEAN, WJ ;

WEBSTER, RG .

JOURNAL OF VIROLOGY, 1991, 65 (10) :5491-5498

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