Significant associations of HLA-B*5101 and B*5108, and lack of association of class II alleles with Behcet's disease in Italian patients

被引:66
作者
Kera, J
Mizuki, N
Ota, M
Katsuyama, Y
Pivetti-Pezzi, P
Ohno, S
Inoko, H [1 ]
机构
[1] Tokai Univ, Sch Med, Dept Genet Informat, Div Mol Life Sci, Isehara, Kanagawa 2591193, Japan
[2] Yokohama City Univ, Sch Med, Dept Ophthalmol, Kanazawa Ku, Yokohama, Kanagawa 232, Japan
[3] Shinshu Univ, Sch Med, Dept Legal Med, Matsumoto, Nagano 390, Japan
[4] Univ La Sapienza, Inst Ophthalmol, Rome, Italy
来源
TISSUE ANTIGENS | 1999年 / 54卷 / 06期
关键词
HLA; Behcet's disease; Italian; B51; genotyping; PCR-SBT;
D O I
10.1034/j.1399-0039.1999.540605.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Behcet's disease has been known to be strongly associated with human leukocyte antigen (HLA) B51, one of the split antigens of HLA-B5, An increased incidence of HLA-B51 in the patient group has also been reported in an Italian population. Since the B51 antigen has been recently identified to comprise nine alleles, B*5101-B*5109, we performed HLA-B51 allele genotyping by the polymerase chain reaction-sequencing based typing . (PCR-SBT) method as well as serological HLA-A and -B typing among 21 Italian patients with Behcets disease in order to investigate whether there is any correlation of one particular B51-associated allele with Behcet's disease. In addition, HLA class II genotyping was performed by the PCR-restriction fragment length polymorphism (RFLP) method. As a result, only the phenotype frequency of the B51 antigen was found to be significantly increased in the patient group as compared to the ethnically matched control group by the corrected P-value analysis (71.4% in patients vs. 17.9% in controls; chi(2) = 14.26, Pc=0.0042, R.R.=11.5), In the B51 allele genotyping, 11 out of 15 B51-positive patients were B*5101 and the remaining four were B*5108, whereas all of 5 normal controls were B*5101, showing significant association of each allele with Behcet's disease. No significant difference was observed between the patient and control groups in the HLA class II allelic distribution. This study revealed a strong association of Behcet's disease in Italian with B*5108 as well as B*5101, providing important insight into the molecular mechanism underlying an FILA association with Behcet's disease.
引用
收藏
页码:565 / 571
页数:7
相关论文
共 35 条
[1]   SEROLOGICAL AND MOLECULAR HLA TYPING IN ITALIAN BEHCET PATIENTS - SIGNIFICANT ASSOCIATION TO B51-DR5-DQW3 HAPLOTYPE [J].
BALBONI, A ;
PIVETTIPEZZI, P ;
ORLANDO, P ;
RUBINI, M ;
SELVATICI, R ;
ACCORINTI, M ;
BARICORDI, OR ;
GANDINI, E .
TISSUE ANTIGENS, 1992, 39 (03) :141-143
[2]  
Barber LD, 1997, J IMMUNOL, V158, P1660
[3]   BEHCETS-DISEASE ASSOCIATED WITH HLA-B51 AND DRW52 ANTIGENS IN ITALIANS [J].
BARICORDI, OR ;
SENSI, A ;
PIVETTIPEZZI, P ;
PERRONE, S ;
BALBONI, A ;
CATARINELLI, G ;
FILIPPI, F ;
MELCHIORRI, L ;
MONCADA, A ;
MATTIUZ, PL .
HUMAN IMMUNOLOGY, 1986, 17 (03) :297-301
[4]   Nomenclature for factors of the HLA system, 1996 [J].
Bodmer, JG ;
Marsh, SGE ;
Albert, ED ;
Bodmer, WF ;
Bontrop, RE ;
Charron, D ;
Dupont, B ;
Erlich, HA ;
Fauchet, R ;
Mach, B ;
Mayr, WR ;
Parham, P ;
Sasazuki, T ;
Schreuder, GMT ;
Strominger, JL ;
Svejgaard, A ;
Terasaki, PI .
TISSUE ANTIGENS, 1997, 49 (03) :297-321
[5]   RAPID ISOLATION OF EUKARYOTIC DNA [J].
BOWTELL, DDL .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (02) :463-465
[6]   Recruitment of tyrosine phosphatase HCP by the killer cell inhibitory receptor [J].
Burshtyn, DN ;
Scharenberg, AM ;
Wagtmann, N ;
Rajagopalan, S ;
Berrada, K ;
Yi, TL ;
Kinet, JP ;
Long, EO .
IMMUNITY, 1996, 4 (01) :77-85
[7]   CLONING OF IMMUNOGLOBULIN-SUPERFAMILY MEMBERS ASSOCIATED WITH HLA-C AND HLA-B RECOGNITION BY HUMAN NATURAL-KILLER-CELLS [J].
COLONNA, M ;
SAMARIDIS, J .
SCIENCE, 1995, 268 (5209) :405-408
[8]   Peripheral blood T cell expansions in patients with Behcet's disease [J].
Esin, S ;
Gul, A ;
Hodara, V ;
JeddiTehrani, M ;
Dilsen, N ;
Konice, M ;
Wigzell, H .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1997, 107 (03) :520-527
[9]   Phosphotyrosines in the killer cell inhibitory receptor motif of NKB1 are required for negative signaling and for association with protein tyrosine phosphatase 1C [J].
Fry, AM ;
Lanier, LL ;
Weiss, A .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (01) :295-300
[10]   THE BW4 PUBLIC EPITOPE OF HLA-B MOLECULES CONFERS REACTIVITY WITH NATURAL-KILLER-CELL CLONES THAT EXPRESS NKB1, A PUTATIVE HLA RECEPTOR [J].
GUMPERZ, JE ;
LITWIN, V ;
PHILLIPS, JH ;
LANIER, LL ;
PARHAM, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 181 (03) :1133-1144