Fabrication and evaluation of 3D β-TCP scaffold by novel direct-write assembly method

Various scaffold fabrication methods have been explored to enhance the cell interaction effects and mechanical properties of scaffolds in bone regeneration. Rapid prototyping (RP) for tissue engineering is a useful technology that may provide a potential scaffolding structure to regenerate, restore, and repair a damaged bone tissue or organ, that is, RP is a promising tissue engineering technique through a 3D scaffold fabrication by using a computer-aided design/computer-aided manufacturing system. In this study, 3D beta-tricalcium phosphate (beta-TCP) scaffolds were fabricated by a novel direct-write assembly method. The mechanical property of beta-TCP scaffolds was analyzed by stress-strain curves by using a compression testing machine. Furthermore, an in vitro CCK-8 assay of osteosarcoma MG-63 cells showed the significant cell attachment and proliferation in the beta-TCP scaffold.