Clinical biomarkers of pulmonary carcinoid tumors in never smokers via profiling miRNA and target mRNA

被引：15

作者：

Deng, Bo ^{[1
,2
]}

Molina, Julian ^{[3
]}

Aubry, Marie C. ^{[4
]}

Sun, Zhifu ^{[1
]}

Wang, Liang ^{[5
]}

Eckloff, Bruce W. ^{[6
]}

Vasmatzis, George ^{[7
]}

You, Ming ^{[8
]}

Wieben, Eric D. ^{[9
]}

Jen, Jin ^{[4
]}

Wigle, Dennis A. ^{[10
]}

Yang, Ping ^{[1
]}

机构：

[1] Mayo Clin, Coll Med, Dept Hlth Sci Res, Rochester, MN 55905 USA

[2] Third Mil Med Univ, Daping Hosp, Inst Surg Res, Thorac Surg Dept, Chongqing, Peoples R China

[3] Mayo Clin, Coll Med, Dept Oncol, Div Med Oncol, Rochester, MN USA

[4] Mayo Clin, Coll Med, Dept Lab Med & Pathol, Rochester, MN USA

[5] Coll Med, Wisconsin Canc Ctr, Dept Pathol, Milwaukee, WI USA

[6] Mayo Clin, Coll Med, Med Genome Facil, Rochester, MN USA

[7] Mayo Clin, Coll Med, Dept Mol Med, Rochester, MN USA

[8] Med Coll Wisconsin, Dept Canc Ctr & Toxicol, Milwaukee, WI 53226 USA

[9] Mayo Clin, Coll Med, Dept Biochem & Mol Biol, Rochester, MN USA

[10] Mayo Clin, Coll Med, Dept Surg, Div Gen Thorac Surg, Rochester, MN USA

来源：

CELL AND BIOSCIENCE | 2014年 / 4卷

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

miRNA; mRNA; Carcinoid; Survival; NEUROENDOCRINE TUMORS; EXPRESSION; GENE; CANCER; LUNG; P53; PROLIFERATION; ASSOCIATION; GROWTH;

D O I：

10.1186/2045-3701-4-35

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: miRNAs play key regulatory roles in cellular pathological processes. We aimed to identify clinically meaningful biomarkers in pulmonary carcinoid tumors (PCTs), a member of neuroendocrine neoplasms, via profiling miRNAs and mRNAs. Results: From the total of 1145 miRNAs, we obtained 16 and 17 miRNAs that showed positive and negative fold changes (FCs, tumors vs. normal tissues) in the top 1% differentially expressed miRNAs, respectively. We uncovered the target genes that were predicted by at least two prediction tools and overlapped by at least one-half of the top miRNAs, which yielded 44 genes (FC<-2) and 56 genes (FC>2), respectively. Higher expressions of CREB5, PTPRB and COL4A3 predicted favorable disease free survival (Hazard ratio: 0.03, 0.19 and 0.36; P value: 0.03, 0.03 and 0.08). Additionally, 79 mutated genes have been found in nine PCTs where TP53 was the only repeated mutation. Conclusion: We identified that the expressions of three genes have clinical implications in PCTs. The biological functions of these biomarkers warrant further studies.

引用

页数：10