New technique to isolate anthocyanins from Delaware grapes by forming an aluminium complex using a Discovery DPA-6S

被引：3

作者：

Asada, Takayuki ^{[1
]}

Koi, Yoriko ^{[2
]}

Tamura, Hirotoshi ^{[1
,2
]}

机构：

[1] Ehime Univ, United Grad Sch Agr Sci, Matsuyama, Ehime 7908566, Japan

[2] Kagawa Univ, Fac Agr, Dept Appl Biol Sci, Miki, Kagawa 7610795, Japan

来源：

FOOD CHEMISTRY | 2015年 / 166卷

关键词：

Anthocyanidin; 3-glucoside; Acylated anthocyanidin 3-glucoside; Complex pigment; Selective isolation; BLUE-COLOR DEVELOPMENT; ANTIOXIDANT ACTIVITY; COMMELINA-COMMUNIS; FLOWER PETALS; SUPRAMOLECULAR PIGMENT; VACCINIUM-MYRTILLUS; CENTAUREA-CYANUS; SALVIA PATENS; METALLOANTHOCYANIN; CORNFLOWER;

D O I：

10.1016/j.foodchem.2014.05.100

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An aluminium complex of crude Delaware grape anthocyanins, bearing an ortho-dihydroxyl group on the B ring and/or a p-coumaroyl group as an ester with flavocommelin, was formed and isolated by ethanol precipitation. Using a Discovery DPA-6S short column, selected anthocyanins were isolated to give cyanidin 3-glucoside (Cy3G, 48.2% yield with 95.2% purity) and cyanidin 3-(6-O-p-coumaroylglucoside) (Cy3-pC.G, 44.9% yield with 91.4% purity) from natural Delaware grape skin extracts without ODS-HPLC column chromatography. DPPH radical scavenging activity of the complex pigment was 3.4 +/- 0.10 mu mol TE/mg. Moreover, isolated pure anthocyanins from the complex pigment showed significantly higher DPPH radical scavenging activity [4.5 +/- 0.08 mu mol TE/mg (Cy3G) and 4.5 +/- 0.04 mu mol TE/mg (Cy3-pC.G), (p < 0.05)]. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：10 / 16

页数：7