DETERMINATION OF GLIBENCLAMIDE, METFORMIN HYDROCHLORIDE AND ROSIGLITAZONE MALEATE BY REVERSED PHASE LIQUID CHROMATOGRAPHIC TECHNIQUE IN TABLET DOSAGE FORM

A simple, precise and accurate high performance liquid chromatography (HPLC) method was developed for the simultaneous estimation of mefformin hydrochloride, rosiglitazone maleate, glibenclamide present in multicomponent dosage forms. Chromatography was performed on a 25 cmx4.6 mm id, 5-mu m particle, C-18 column with 78:22 (v/v) methano1:20 mM potassium dihydrogen phosphate buffer as mobile phase at a flow rate of 1.0 ml/min and UV detection at 238 nm for metformin hydrochloride, rosiglitazone maleate and glibenclamide. The total elution time was shorter than 9 min. This method was found to be precise and reproducible. The proposed method was successfully applied for the analysis of mefformin hydrochloride, rosiglitazone maleate, glibenclamide as a bulk drug and in pharmaceutical formulation without any interference from the excipients.