Cloning and characterization of two members of the chalcone synthase gene family from walnut

被引:19
作者
Claudot, AC
Ernst, D [1 ]
Sandermann, H
Drouet, A
机构
[1] GSF, Natl Res Ctr Environm & Hlth, Inst Biochem Plant Pathol, D-85764 Neuherberg, Germany
[2] Univ Orleans, Lab Physiol Vegetale, F-45067 Orleans 2, France
关键词
chalcone synthase; flavonoids; gene expression; Juglans; promoter; rejuvenation;
D O I
10.1016/S0981-9428(00)86685-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Based on PCR technology two full length chalcone synthase (CHS) cDNAs with about 98 % identity at the nucleic acid level between each other were isolated from leaves of adult walnut tree. The open reading frame sequences coded for two 389-amino acid polypeptides with 99.5 % identity. The two deduced amino acid sequences exhibited the typical CHS consensus in the middle of the sequences and the essential amino acids for CHS (EC 2.3.1.74) activity; in addition, they showed a high degree of identity to the CHS of Pyrus malus (91 %) and Pinus sylvestris (85 %). Perfect repeats were detected in the single intron of one genomic CHS clone isolated. Southern blot analysis indicated that in walnut, CHS is encoded by a small gene family. Sequence analysis of the promoter region of one member of the CHS gene family revealed putative cis-regulatory elements, such as a G-box, H-box and W-box. Accumulation of a 15-kb CHS transcript was detected in leaves, buds, liber and bark, whereas no transcripts were detected in wood and medulla. Growth of walnut shoots resulted in a transient increase of leaf and liber CHS transcripts which was much more pronounced in adult than in rejuvenated tree shoots. These data which are correlated with CHS enzyme activity and flavonoid accumulation indicate a tissue-specific, as well as an influence caused by old age on flavonoid biosynthesis, controlled at the transcriptional level. (C) 1999 Editions scientifiques et medicales Elsevier SAS.
引用
收藏
页码:721 / 730
页数:10
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