A facile strategy to functionalize gold nanorods with polycation brushes for biomedical applications

被引：36

作者：

Yan, Peng ^{[1
]}

Zhao, Nana ^{[1
]}

Hu, Hao ^{[1
]}

Lin, Xinyi ^{[1
]}

Liu, Fusheng ^{[2
]}

Xu, Fu-Jian ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Key Lab Carbon Fiber & Funct Polymers, State Key Lab Chem Resource Engn,Minist Educ, Beijing Lab Biomed Mat,Coll Mat Sci & Engn, Beijing 100029, Peoples R China

[2] Capital Med Univ, Beijing Tiantan Hosp, Beijing Neurosurg Inst, Brain Tumor Res Ctr, Beijing 100050, Peoples R China

来源：

ACTA BIOMATERIALIA | 2014年 / 10卷 / 08期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Gold nanorods; Polycation; ATRP; Gene carriers; CT imaging; TRANSFER RADICAL POLYMERIZATION; BETA-CYCLODEXTRIN CORES; X-RAY ATTENUATION; CATIONIC POLYMERS; CELLULAR UPTAKE; NANOPARTICLES; SIZE; METHACRYLATE); NANOHYBRIDS; COPOLYMERS;

D O I：

10.1016/j.actbio.2014.05.002

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The fabrication of highly efficient nonviral gene carriers with low cytotoxicity remains a challenge in gene therapy. This paper reports a facile strategy to combine the advantages of gold nanorods (Au NRs) and polycations through surface functionalization. Different Au NR carriers with a controlled amount of poly(2-(N,N-dimethyl amino)ethyl methacrylate) (PDAEMA) brushes could be readily synthesized via surface-initiated atom transfer radical polymerization to achieve optimized nanohybrids for gene transfection. The obtained gene carriers demonstrate much higher gene transfection efficiency and lower cytotoxicity compared with polyethylenimine (similar to 25 kDa, gold standard of nonviral gene vector) in both COS7 and HepG2 cell lines. In addition, the potential of the PDMAEMA-grafted Au NR carriers to be utilized as a computed tomography contrast agent for the imaging of cancer cells has also been investigated. This strategy may realize the gene therapy and real-time imaging within one nanostructure and facilitate biomedical applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：3786 / 3794

页数：9

共 38 条

[1] Well-defined hydroxyapatite-polycation nanohybrids via surface-initiated atom transfer radical polymerization for biomedical applications [J].