GENETIC DIVERSITY OF SYMPLOCOS PANICULATA OF HUNAN PROVINCE REVEALED BY INTER-SIMPLE SEQUENCE REPEAT (ISSR)

The natural populations' genetic diversity of Symplocos paniculata in Hunan province was evaluated by using Inter-simple sequence repeat (ISSR) markers. In all 122 loci were identified in 149 individual plants of six populations using nine selected ISSR primers (UBC815, UBC817, UBC824, UBC835, UBC845, UBC853, UBC862, UBC873 and UBC879) and 92.2% loci were polymorphic. Nei's gene diversity (H) and shannon information index (I) were 0.3264 and 0.4873, respectively. The coefficient of gene differentiation (Gst) was 0.5397. Gene flow among populations (Nm) was 0.4763. Analysis of molecular variance (AMOVA) demonstrated that the genetic differentiation among S. paniculata populations was relatively high (p<0.001). The among-population component accounted for 51.1% of the total variation, while the within-population component accounted for 49.8%. Six populations were clustered into three groups by the unweighted pair-group method arithmetic average (UPGMA). The results showed that populations of Da Wei Shan A (DWSA), Da Wei Shan B (DWSB) and Xiang Xi Long Shan (XXLS) were in the first group, populations of Yue Yang Jun Shan (YYJS) and Yong Zhou Dao Xian (YZDX) were in another group, and Heng Yang Hen Shan (HYHS) population separated from all the populations in the dendrogram. The cluster results were mutually related to the geographical elevation, but not to geographical distribution.