Isolation and structural analysis of an acidic polysaccharide from Astragalus membranaceus (Fisch.) Bunge

被引：16

作者：

Wang, Shun-Chun ^{[1
]}

Shan, Jun-Jie ^{[1
]}

Wang, Zheng-Tao ^{[1
]}

Hu, Zhi-Bi ^{[1
]}

机构：

[1] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2006年 / 48卷 / 11期

关键词：

Astragalus membranaceus; hetero-polysaccharide; isolation and purification; structural analysis;

D O I：

10.1111/j.1744-7909.2006.00325.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new water-soluble hetero-polysaccharide, APSID3, was obtained from a hot-water extract of the roots of Astragalus membranaceus (Fisch.) Bunge by DEAE-Sepharose Fast Flow and Sephacryl S-300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 x 10(5)Da. Based on a sugar composition analysis, methylation analysis, partial hydrolysis and C-13 nuclear magnetic resonance experimentation, it was concluded that the minimal repeat unit of APSID3 was composed of one terminal arabinose, one 1,5-linked arabinose, one 1,3-linked rhamnose, one 1,3,4-linked rhamnose, five 1,4-linked methyl galacturonates and six 1,4-linked methyl glucuronates.

引用

页码：1379 / 1384

页数：6

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