Cryopreservation of porcine embryos derived from in vitro-matured oocytes

被引：75

作者：

Esaki, R ^{[1
]}

Ueda, H ^{[1
]}

Kurome, M ^{[1
]}

Hirakawa, K ^{[1
]}

Tomii, R ^{[1
]}

Yoshioka, H ^{[1
]}

Ushijima, H ^{[1
]}

Kuwayama, M ^{[1
]}

Nagashima, H ^{[1
]}

机构：

[1] Meiji Univ, Sch Agr, Dept Life Sci, Lab Dev Engn, Kawasaki, Kanagawa 2148571, Japan

来源：

BIOLOGY OF REPRODUCTION | 2004年 / 71卷 / 02期

关键词：

embryo; gamete biology; developmental biology; early development; ovum;

D O I：

10.1095/biolreprod.103.026542

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study describes a cryo preservation method for porcine in vitro-produced (IVP) embryos using as a model parthenogenetic embryos derived from in vitro-matured (IVM) oocytes. IVP embryos at the expanded blastocyst stage were cryopreserved by vitrification using the minimum volume cooling (MVC) method and exhibited an embryo survival rate of 41.2%. Survival was then significantly improved (83.3%, P < 0.05) by decreasing the amount of cytoplasmic lipid droplets (delipation) prior to vitrification. IVP embryos at the 4-cell stage also survived cryopreservation when vitrified after delipation (survival rate, 36.0%), whereas post-thaw survival of nondelipated embryos was quite low (9.7%). Furthermore, it was demonstrated that porcine IVP morulae can be cryopreserved by vitrification following delipation by a noninvasive method (survival rate, 82.5%). These results clearly confirm that porcine embryos derived from IVM oocytes can be effectively cryopreserved with high embryo survival using the MVC method in conjunction with delipation.

引用

页码：432 / 437

页数：6