Nucleosome-free DNA regions differentially affect distant communication in chromatin

被引：20

作者：

Nizovtseva, Ekaterina V. ^{[1
,2
]}

Clauvelin, Nicolas ^{[3
]}

Todolli, Stefjord ^{[3
]}

Polikanov, Yury S. ^{[2
,6
]}

Kulaeva, Olga I. ^{[1
,2
,4
]}

Wengrzynek, Scott ^{[2
]}

Olson, Wilma K. ^{[3
]}

Studitsky, Vasily M. ^{[1
,5
]}

机构：

[1] Fox Chase Canc Ctr, Canc Epigenet Program, 333 Cottman Ave, Philadelphia, PA 19422 USA

[2] Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Pharmacol, 675 Hoes Lane, Piscataway, NJ 08854 USA

[3] Rutgers State Univ, Ctr Quantitat Biol, Dept Chem & Chem Biol, 610 Taylor Rd, Piscataway, NJ 08854 USA

[4] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia

[5] Russian Acad Sci, Inst Gene Biol, Lab Epigenet, Moscow, Russia

[6] Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA

来源：

NUCLEIC ACIDS RESEARCH | 2017年 / 45卷 / 06期

基金：

俄罗斯科学基金会;

关键词：

HIGHER-ORDER STRUCTURE; CORE PARTICLE; ENHANCER ACTION; FIBER; PROTEIN; TRANSCRIPTION; PROMOTERS; ACTIVATOR; POLYMORPHISM; ASSOCIATION;

D O I：

10.1093/nar/gkw1240

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleosome spacing and the presence of nucleosome-free DNA regions can modulate chromatin structure/dynamics and, in turn, affect the rate of EPC in vitro and in silico. Increasing the length of internucleosomal linker DNA from 25 to 60 bp results in more efficient EPC. The presence of longer nucleosome-free DNA regions can positively or negatively affect the rate of EPC, depending upon the length and location of the DNA region within the chromatin fiber. Thus the presence of histone-free DNA regions can differentially affect the efficiency of EPC, suggesting that gene regulation over a distance could be modulated by changes in the length of internucleosomal DNA spacers.

引用

页码：3059 / 3067

页数：9

共 53 条

[1] THE NUCLEOSOMAL CORE HISTONE OCTAMER AT 3.1-A RESOLUTION - A TRIPARTITE PROTEIN ASSEMBLY AND A LEFT-HANDED SUPERHELIX [J].