The Effects of radiation on the safety and protective efficacy of an attenuated Plasmodium yoelii sporozoite malaria vaccine

被引：37
作者：
Chattopadhyay, Rana [1 ]
Conteh, Solomon [1 ]
Li, MingLin [1 ,2 ]
James, Eric R. [1 ]
Epstein, Judith E. [2 ]
Hoffman, Stephen L. [1 ,3 ]
机构：
[1] Sanaria Inc, Rockville, MD 20850 USA
[2] Prot Potential LLC, Rockville, MD 20850 USA
[3] USN, Med Res Ctr, US Mil Malaria Vaccine Program, Silver Spring, MD 20910 USA
来源：
VACCINE | 2009年 / 27卷 / 27期
关键词：
Malaria; Vaccine; Sporozoites; Radiation; Attenuation; Plasmodium yoelii; IRRADIATED SPOROZOITES; FALCIPARUM SPOROZOITES; SURFACE PROTEIN-2; VIVAX MALARIA; IMMUNIZATION; IMMUNITY; ANTIGEN; STRAIN; LEMURINUS; INFECTION;
D O I：
10.1016/j.vaccine.2008.11.073
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We are developing a radiation attenuated Plasmodium falciparum sporozoite (PfSPZ) malaria vaccine. An important step was to determine the minimum dose of irradiation required to adequately attenuate each sporozoite. This was studied in the Plasm odium yoelii rodent model system. Exposure to 100 Gy completely attenuated P. yoelii sporozoites (PySPZ). Next we demonstrated that immunization of mice intravenously with 3 doses of 750 PySPZ that had received 200 Gy, double the radiation dose required for attenuation, resulted in 100% protection. These results support the contention that a radiation attenuated sporozoite vaccine for malaria will be safe and effective at a range of radiation doses. (C) 2008 Elsevier Ltd. All rights reserved.
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收藏
页码：3675 / 3680
页数：6
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