Focused Ion Beam-based Milling, Imaging and Analysis of 3D Tumor Spheroids

被引：0

作者：

Almarshad, Hassan A. ^{[1
]}

Madhavan, Mathangi ^{[1
]}

Hoshino, Kazunori ^{[2
]}

机构：

[1] Univ Connecticut, Storrs, CT 06269 USA

[2] Univ Connecticut, Biomed Engn Dept, Storrs, CT 06269 USA

来源：

2018 40TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2018年

关键词：

DELIVERY; CELLS;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We investigate the structural cellular alterations in breast cancer spheroids at various growth stages using transmission electron microscopy (TEM), focused ion beam (FIB), and scanning electron microscopy (SEM) imaging. Samples sliced by FIB milling were studied for 3D analysis and construction. The imaging results of different spheroid ages were compared for a better understanding of cancer spheroid models. This study will serve as a pilot study and reference control for further studies with the 3D tumor model including nanoparticles interaction and mechanical characterization.

引用

页码：4480 / 4483

页数：4

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