Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells

被引：44
作者：
Park, Jung-Eun [1 ]
Cruz, Deu John M. [1 ]
Shin, Hyun-Jin [1 ,2 ]
机构：
[1] Coll Vet Med, Infect Dis Lab, Seoul, South Korea
[2] Chungnam Natl Univ, Vet Med Res Inst, Taejon 305764, South Korea
来源：
VIRUS RESEARCH | 2014年 / 191卷
关键词：
Porcine epidemic diarrhea; Coronavirus; Entry; Clathrin; Low pH; Serine protease; TRANSMISSIBLE GASTROENTERITIS VIRUS; INFECTIOUS PERITONITIS VIRUS; CORONAVIRUS SPIKE PROTEIN; CATHEPSIN-L; SARS CORONAVIRUS; AMINOPEPTIDASE-N; MEMBRANE-FUSION; ENTRY; ENDOCYTOSIS; RECEPTOR;
D O I：
10.1016/j.virusres.2014.07.022
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells. (C) 2014 Elsevier B.V. All rights reserved.
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页码：21 / 29
页数：9
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