Anaplasma phagocytophilum groEL Gene Heterogeneity in Ixodes ricinus Larvae Feeding on Roe Deer in Northeastern Italy

被引：42

作者：

Carpi, Giovanna ^{[1
]}

Bertolotti, Luigi ^{[2
]}

Pecchioli, Elena ^{[1
]}

Cagnacci, Francesca ^{[1
]}

Rizzoli, Annapaola ^{[1
]}

机构：

[1] Edmund Mach Fdn, Ctr Alpine Ecol, I-38100 Trento, Italy

[2] Univ Turin, Fac Vet Med, Dipartimento Produz Anim, Grugliasco, TO, Italy

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2009年 / 9卷 / 02期

关键词：

Anaplasma; Epidemiology; Genetics; Ixodes; HUMAN GRANULOCYTIC EHRLICHIOSIS; CAPREOLUS-CAPREOLUS; WILD ANIMALS; EUROPE; INFECTION; PCR; DISEASE; TICKS; SUBSTITUTION; PREVALENCE;

D O I：

10.1089/vbz.2008.0068

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Anaplasma phagocytophilum is an emerging tick-borne pathogen with both veterinary and human health implications. The role of wildlife hosts for this pathogen are not well defined, even thought roe deer (Capreolus capreolus) has been suggested to contribute to the occurrence of this tick-borne diseases in Europe. Therefore the aim of the present study was to investigate the potential role of this ungulate species as a reservoir of human pathogenic strains of A. phagocytophilum in a tick-borne diseases endemic area in Northeastern Italy. Ixodes ricinus feeding on roe deer were collected and analyzed for the presence for A. phagocytophilum by a molecular approach targeting 16S rRNA and groEL genes. The mean prevalence of A. phagocytophilum recorded was 5.11%, highlighting the ability of roe deer to infect the I. ricinus larval stage. The results of further genetic characterization of the strains of A. phagocytophilum herein isolated, based on phylogenetic information contained in groEL gene sequences, showed substantial heterogeneity among sequences analyzed. Nevertheless, these findings suggest that the roe deer population of the Trentino region of Italy harbors strains of A. phagocytophilum of unknown pathogenicity for humans.

引用

页码：179 / 184

页数：6

共 43 条

[1]

Akaike H., 1973, 2 INT S INFORM THEOR, P267

[2] Field evidence that roe deer (Capreolus capreolus) are a natural host for Ehrlichia phagocytophila [J].

Alberdi, MP ;

Walker, AR ;

Urquhart, KA .

EPIDEMIOLOGY AND INFECTION, 2000, 124 (02) :315-323

[3] Equine and canine Anaplasma phagocytophilum strains isolated on the island of Sardinia (Italy) are phylogenetically related to pathogenic strains from the United States [J].

Alberti, A ;

Zobba, R ;

Chessa, B ;

Addis, MF ;

Sparagano, O ;

Parpaglia, MLP ;

Cubeddu, T ;

Pintori, G ;

Pittau, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (10) :6418-6422

[4]

[Anonymous], 2003, PAUP PHYLOGENETIC AN

[5] Human granulocytic anaplasmosis in Northeastern Italy [J].

Beltrame, Anna ;

Ruscio, Maurizio ;

Arzese, Alessandra ;

Rorato, Giada ;

Negri, Camilla ;

Londero, Angela ;

Crapis, Massimo ;

Scudeller, Luigia ;

Viale, Pierluigi .

CENTURY OF RICKETTSIOLOGY: EMERGING, REEMERGING RICKETTSIOSES, MOLECULAR DIAGNOSTICS, AND EMERGING VETERINARY RICKETTSIOSES, 2006, 1078 :106-109

[6] Anaplasmataceae in wild rodents and roe deer from Trento Province (northern Italy) [J].

Beninati T. ;

Piccolo G. ;

Rizzoli A. ;

Genchi C. ;

Bandi C. .

European Journal of Clinical Microbiology and Infectious Diseases, 2006, 25 (10) :677-678

[7] Human granulocytic ehrlichiosis in Europe [J].

Blanco, JR ;

Oteo, JA .

CLINICAL MICROBIOLOGY AND INFECTION, 2002, 8 (12) :763-772

[8] Tick infestation on roe deer in relation to geographic and remotely sensed climatic variables in a tick-borne encephalitis endemic area [J].

Carpi, G. ;

Cagnacci, F. ;

Neteler, M. ;

Rizzoli, A. .

EPIDEMIOLOGY AND INFECTION, 2008, 136 (10) :1416-1424

[9]

Chemini C., 1997, Parassitologia (Rome), V39, P59

[10] Comparison of methods for estimation of individual-level prevalence based on pooled samples [J].

Cowling, DW ;

Gardner, IA ;

Johnson, WO .

PREVENTIVE VETERINARY MEDICINE, 1999, 39 (03) :211-225

← 1 2 3 4 5 →