Evaluation and comparison of the commonly used bioassays of human indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO)

被引：0

作者：

Zhang, Shengnan ^{[1
]}

Guo, Leilei ^{[1
]}

Yang, Dan ^{[1
]}

Xing, Zikang ^{[1
]}

Li, Weirui ^{[1
]}

Kuang, Chunxiang ^{[2
]}

Yang, Qing ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Dept Biochem, State Key Lab Genet Engn, Songhu Rd 2005, Shanghai 200438, Peoples R China

[2] Tongji Univ, Sch Chem Sci & Engn, Shanghai Key Lab Chem Assessment & Sustainabil, 1239 Siping Rd, Shanghai 200092, Peoples R China

来源：

BIOORGANIC CHEMISTRY | 2020年 / 104卷

基金：

国家重点研发计划;

关键词：

Indoleamine; 2; 3-dioxygenase; 1; Tryptophan; Bioassay; Inhibitor; KYNURENINE METABOLISM; POTENT INHIBITORS; DISCOVERY; DERIVATIVES; CANCER; INDOLEAMINE-2,3-DIOXYGENASE; DESIGN; IDENTIFICATION; PURIFICATION; TARGET;

D O I：

10.1016/j.bioorg.2020.104348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibitors of indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) are potential drugs for the treatment of tumor and neurological diseases. A variety of bioassays have been developed to evaluate IDO1/TDO (IDO1 and/or TDO) inhibitors, with uncertainty regarding how the differences in the assay methods or protocols may influence the assay outcomes. The enzymatic assays of IDO1/TDO are usually performed with NFK assay and Kyn adduct assay while the cellular assays of IDO1 are carried out with Hela assay and HEK293 assay. The present study focused on the comparison of the most common bioassays of IDO1/TDO. In addition, the effects of major factors of bioassays such as reaction time and culture medium on the assay outcomes were evaluated. The study will provide reference for the researchers to select IDO1/TDO inhibitors with bioassays, and promote the development of IDO1/TDO inhibitors.

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页数：10

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