Productivity of cyclohexanone oxidation of the recombinant Corynebacterium glutamicum expressing chnB of Acinetobacter calcoaceticus

被引：26

作者：

Doo, Eun-Hee ^{[1
]}

Lee, Won-Heong ^{[2
,3
]}

Seo, Hyo-Seel ^{[1
]}

Seo, Jin-Ho ^{[2
,3
]}

Park, Jin-Byung ^{[1
]}

机构：

[1] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 120750, South Korea

[2] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea

[3] Seoul Natl Univ, Ctr Agr Biomat, Seoul 151921, South Korea

来源：

JOURNAL OF BIOTECHNOLOGY | 2009年 / 142卷 / 02期

关键词：

Biocatalysis; Baeyer-Villiger oxidation; Corynebacterium glutamicum; Cyclohexanone monooxygenase; epsilon-Caprolactone; BAEYER-VILLIGER OXIDATION; ESCHERICHIA-COLI; CENTRAL METABOLISM; MONOOXYGENASE; BIOCATALYST; FLUXES; CELLS; GENE;

D O I：

10.1016/j.jbiotec.2009.04.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biocatalytic efficiency of recombinant Corynebacterium glutamicum expressing the chnB gene encoding cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871 was investigated. Optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce CHMO to a specific activity of ca. 0.5 U mg(-1) protein. Tight control of feeding of an energy source (i.e., glucose) and dissolved oxygen tension during fed-batch culture-based biotransformation allowed the cells to produce epsilon-caprolactone to a concentration of 16.0 gl(-1). The specific and volumetric productivity for cyclohexanone oxidation were 0.12 g g dry cell s(-1)h(-1) (17.5 Ug(-1) of dry cells) and 2.3 gl(-1) h(-1) (330 Ul(-1)), respectively. These values correspond to over 5.4- and 2.7-fold of recombinant Escherichia call expressing the same gene under similar reaction conditions. It could be concluded that the recombinant C. glutamicum is a promising biocatalyst for Baeyer-Villiger oxidations. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：164 / 169

页数：6

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