The TRANSPORT INHIBITOR RESPONSE2 Gene Is Required for Auxin Synthesis and Diverse Aspects of Plant Development

被引:147
作者
Yamada, Masashi [1 ]
Greenham, Katie [1 ]
Prigge, Michael J. [1 ]
Jensen, Philip J. [1 ]
Estelle, Mark [1 ]
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
基金
美国国家卫生研究院;
关键词
BOX PROTEIN TIR1; ARABIDOPSIS-THALIANA; INDOLE-3-ACETIC-ACID BIOSYNTHESIS; INDOLE-3-PYRUVIC ACID; ROOT GRAVITROPISM; TRANSPORT; GROWTH; TRYPTOPHAN; ETHYLENE; AMINOTRANSFERASE;
D O I
10.1104/pp.109.138859
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plant hormone auxin plays an essential role in plant development. However, only a few auxin biosynthetic genes have been isolated and characterized. Here, we show that the TRANSPORT INHIBITOR RESPONSE2 (TIR2) gene is required for many growth processes. Our studies indicate that the tir2 mutant is hypersensitive to 5-methyl-tryptophan, an inhibitor of tryptophan synthesis. Further, treatment with the proposed auxin biosynthetic intermediate indole-3-pyruvic acid (IPA) and indole-3-acetic acid rescues the tir2 short hypocotyl phenotype, suggesting that tir2 may be affected in the IPA auxin biosynthetic pathway. Molecular characterization revealed that TIR2 is identical to the TAA1 gene encoding a tryptophan aminotransferase. We show that TIR2 is regulated by temperature and is required for temperature-dependent hypocotyl elongation. Further, we find that expression of TIR2 is induced on the lower side of a gravitropically responding root. We propose that TIR2 contributes to a positive regulatory loop required for root gravitropism.
引用
收藏
页码:168 / 179
页数:12
相关论文
共 53 条
[1]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[2]   NOVEL INHIBITORS OF ETHYLENE PRODUCTION IN HIGHER-PLANTS [J].
AMRHEIN, N ;
WENKER, D .
PLANT AND CELL PHYSIOLOGY, 1979, 20 (08) :1635-1642
[3]   Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].
Benková, E ;
Michniewicz, M ;
Sauer, M ;
Teichmann, T ;
Seifertová, D ;
Jürgens, G ;
Friml, J .
CELL, 2003, 115 (05) :591-602
[4]   A gene expression map of the Arabidopsis root [J].
Birnbaum, K ;
Shasha, DE ;
Wang, JY ;
Jung, JW ;
Lambert, GM ;
Galbraith, DW ;
Benfey, PN .
SCIENCE, 2003, 302 (5652) :1956-1960
[5]   The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots [J].
Blilou, I ;
Xu, J ;
Wildwater, M ;
Willemsen, V ;
Paponov, I ;
Friml, J ;
Heidstra, R ;
Aida, M ;
Palme, K ;
Scheres, B .
NATURE, 2005, 433 (7021) :39-44
[6]   The Arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier [J].
Chen, RJ ;
Hilson, P ;
Sedbrook, J ;
Rosen, E ;
Caspar, T ;
Masson, PH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :15112-15117
[7]   Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis [J].
Cheng, Youfa ;
Dai, Xinhua ;
Zhao, Yunde .
GENES & DEVELOPMENT, 2006, 20 (13) :1790-1799
[8]  
COONEY TP, 1991, PLANTA, V184, P368, DOI 10.1007/BF00195339
[9]   A gateway cloning vector set for high-throughput functional analysis of genes in planta [J].
Curtis, MD ;
Grossniklaus, U .
PLANT PHYSIOLOGY, 2003, 133 (02) :462-469
[10]  
Davies P, 1995, PLANT HORMONES PHYSL