Immunophenotyping of mature B-cell non Hodgkin lymphoma involving bone marrow and peripheral blood: critical analysis and insights gained at a tertiary care cancer hospital

被引：15

作者：

Gujral, Sumeet ^{[1
]}

Polampalli, Sunita Narayan ^{[2
]}

Badrinath, Y. ^{[4
]}

Kumar, Ashok ^{[4
]}

Subramanian, P. G. ^{[4
]}

Nair, Reena ^{[3
]}

Gupta, Sudeep ^{[3
]}

Sengar, Manju ^{[3
]}

Nair, Chandralekha ^{[3
]}

机构：

[1] Tata Mem Hosp, Dept Pathol, Bombay 400080, Maharashtra, India

[2] Tata Mem Hosp, Dept Mol Biol, Bombay 400080, Maharashtra, India

[3] Tata Mem Hosp, Dept Med Oncol, Bombay 400080, Maharashtra, India

[4] Tata Mem Hosp, Dept Hematopathol, Bombay 400080, Maharashtra, India

来源：

LEUKEMIA & LYMPHOMA | 2009年 / 50卷 / 08期

关键词：

Mature B cell non Hodgkin lymphoma; immunophenotyping; flow cytometry; CHRONIC LYMPHOCYTIC-LEUKEMIA; FOLLICULAR LYMPHOMA; FLOW-CYTOMETRY; DIAGNOSTIC USEFULNESS; EXPRESSION PATTERNS; SCORING SYSTEM; CLASSIFICATION; CD10; CD5; CLL;

D O I：

10.1080/10428190903064824

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We evaluated the diagnostic utility of flow cytometry immunophenotyping in bone marrow aspirates and peripheral blood, in the assessment of mature B-cell non-Hodgkin lymphoma (MBNHL). We analyzed 356 cases of MBNHL received for immunophenotyping over a 4 year period. All cases were reviewed, correlated with biopsy specimen (lymph node and splenectomy). Discrepant cases were re-evaluated. Common subtypes included chronic lymphocytic leukemia (CLL) (243 cases, 68.5%), follicular lymphoma (30 cases, 8.5%), mantle cell lymphoma (20 cases, 5.5%), splenic marginal zone lymphoma (18 cases, 5%), hairy cell leukemia (18 cases, 5%). CD5+/CD23+ had a high positive predictive value (PPV) for diagnosing CLL whereas CD5+/CD23- had a high negative predictive value (NPV) for diagnosing mantle-cell lymphoma (MCL). Limited panel of 9 antibodies mainly CD19, CD5, CD23, CD10, FMC7, kappa, lambda, CD3 and CD20 help diagnose more than 92% of cases of MBNHL. Minimal diagnostic panels become important in countries with limited resources.

引用

页码：1290 / 1300

页数：11

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