Effects of Cyanidin-3-O-glucoside on Synthetic and Metabolic Activity of Ethanol Stimulated Human Pancreatic Stellate Cells

被引:2
作者
Cesna, Vaidotas [1 ,2 ]
Baniene, Rasa [3 ]
Maziukiene, Aurelija [2 ]
Kmieliute, Kristina [2 ]
Trumbeckaite, Sonata [3 ]
Venclauskas, Linas [1 ,2 ]
Barauskas, Giedrius [1 ]
Gulbinas, Antanas [1 ,2 ]
机构
[1] Lithuanian Univ Hlth Sci, Dept Surg, LT-50009 Kaunas, Lithuania
[2] Lithuanian Univ Hlth Sci, Inst Digest Res, Kaunas, Lithuania
[3] Lithuanian Univ Hlth Sci, Inst Neurosci, Kaunas, Lithuania
关键词
pancreatic stellate cells; cyanidin-3-O-glucoside; ethanol; energy metabolism; ALCOHOLIC PANCREATITIS; ENDOTHELIAL-CELLS; AUTOCRINE LOOP; FIBROSIS; CANCER; IDENTIFICATION; PROLIFERATION; STABILIZATION; ANTHOCYANINS; ACTIVATION;
D O I
10.1002/ptr.5476
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Activated pancreatic stellate cells (PSC) play a major role in the development of chronic pancreatitis. Flavonoids (C-3-O-G) theoretically may have potential to suppress activated PSC. The aim of our study was to determine the ability of C-3-O-G to invert synthetic and metabolic activity of alcohol stimulated human pancreatic stellate cells (hPSC). In the present study we demonstrate that treatment with C-3-O-G decreased proliferation rate of ethanol activated hPSC by 51%. Synthesis of extracellular matrix proteins in activated hPSC was markedly inhibited, as shown by reduced levels of collagen I and fibronectin expression. The decrease of secretion of fibronectin by 33% and in collagen I-25% in ethanol activated and C-3-O-G treated hPSC was observed. Moreover, treatment of ethanol activated hPSC with C-3-O-G resulted in the decrease of oxygen consumption rate by 44% and reduced levels of ATP synthesis (i.e. energy production) by 41%. Hence, the effects of C-3-O-G on ethanol activated hPSC may provide new insights for the use of anthocyanins as anti-fibrogenic agents in treatment and/or prevention of pancreatic fibrosis. Copyright (C) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码:1894 / 1900
页数:7
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