Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria

被引:11
作者
Seralathan, Muhil Vannan [1 ]
Sivanesan, Saravanadevi [1 ]
Nargunanathan, Srinivasan [1 ]
Bafana, Amit [1 ]
Kannan, Krishnamurthi [1 ]
Chakrabarti, Tapan [1 ]
机构
[1] Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
关键词
kinetics; endosulfan; chemotaxis; DGGE; biotransformation; RALSTONIA SP SJ98; PSEUDOMONAS-AERUGINOSA; RHODOBACTER-SPHAEROIDES; BIODEGRADATION; SOIL; BIOREMEDIATION; NAPHTHALENE; STRAIN; IDENTIFICATION; INVOLVEMENT;
D O I
10.1080/09593330.2014.937464
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The study was conducted to isolate endosulfan biotransforming or biodegrading microbes based on chemotaxis. Pseudomonas aeruginosa strain KKc3, Ochrobactrum sp. strain KKc4, Achromobacter xylosoxidans strain KKc6 and Bacillus megaterium KKc7 were isolated based on their migration towards endosulfan in a soil column. Out of the four bacteria, B. megaterium converted endosulfan into toxic metabolite endosulfan sulphate, while the other three bacteria followed the non-toxic endosulfan diol pathway. The mixed culture system consisting of P. aeruginosa, Ochrobactrum sp and A. xylosoxidans could remove 94% of total endosulfan by using endosulfan as the sole source of sulphur.
引用
收藏
页码:60 / 67
页数:8
相关论文
共 43 条
[11]   MOTILITY AND CHEMOTAXIS OF 3 STRAINS OF PSEUDOMONAS-AERUGINOSA USED FOR VIRULENCE STUDIES [J].
CRAVEN, RC ;
MONTIE, TC .
CANADIAN JOURNAL OF MICROBIOLOGY, 1981, 27 (04) :458-460
[12]   CHEMOTAXIS OF PSEUDOMONAS-AERUGINOSA - INVOLVEMENT OF METHYLATION [J].
CRAVEN, RC ;
MONTIE, TC .
JOURNAL OF BACTERIOLOGY, 1983, 154 (02) :780-786
[13]   Chemotaxis of Pseudomonas spp. to the polyaromatic hydrocarbon naphthalene [J].
Grimm, AC ;
Harwood, CS .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (10) :4111-4115
[14]   Evidence for two chemosensory pathways in Rhodobacter sphaeroides [J].
Hamblin, PA ;
Maguire, BA ;
Grishanin, RN ;
Armitage, JP .
MOLECULAR MICROBIOLOGY, 1997, 26 (05) :1083-1096
[15]   Characterization of the chemotaxis protein CheW from Rhodobacter sphaeroides and its effect on the behaviour of Escherichia coli [J].
Hamblin, PA ;
Bourne, NA ;
Armitage, JP .
MOLECULAR MICROBIOLOGY, 1997, 24 (01) :41-51
[16]   Bacterial Chemotaxis along Vapor-Phase Gradients of Naphthalene [J].
Hanzel, Joanna ;
Harms, Hauke ;
Wick, Lukas Y. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2010, 44 (24) :9304-9310
[17]   IDENTIFICATION OF THE PCARKF GENE-CLUSTER FROM PSEUDOMONAS-PUTIDA - INVOLVEMENT IN CHEMOTAXIS, BIODEGRADATION, AND TRANSPORT OF 4-HYDROXYBENZOATE [J].
HARWOOD, CS ;
NICHOLS, NN ;
KIM, MK ;
DITTY, JL ;
PARALES, RE .
JOURNAL OF BACTERIOLOGY, 1994, 176 (21) :6479-6488
[18]  
Hlozkova K, 2013, RES MICROBIOL
[19]   Nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pA81 from Achromobacter xylosoxidans A8 [J].
Jencova, Vera ;
Strnad, Hynek ;
Chodora, Zdenek ;
Ulbrich, Pavel ;
Vlcek, Cestmir ;
Hickey, W. J. ;
Paces, Vaclav .
RESEARCH IN MICROBIOLOGY, 2008, 159 (02) :118-127
[20]   Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene [J].
Kim, Hye-Eun ;
Shitashiro, Maiko ;
Kuroda, Akio ;
Takiguchi, Noboru ;
Ohtake, Hisao ;
Kato, Junichi .
JOURNAL OF BACTERIOLOGY, 2006, 188 (18) :6700-6702