Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria
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Seralathan, Muhil Vannan
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Seralathan, Muhil Vannan
[1
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Sivanesan, Saravanadevi
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Sivanesan, Saravanadevi
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Nargunanathan, Srinivasan
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Nargunanathan, Srinivasan
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Bafana, Amit
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Bafana, Amit
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Kannan, Krishnamurthi
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Kannan, Krishnamurthi
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Chakrabarti, Tapan
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Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, IndiaNatl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
Chakrabarti, Tapan
[1
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机构:
[1] Natl Environm Engn Res Inst, Div Environm Hlth, Nagpur 440022, Maharashtra, India
The study was conducted to isolate endosulfan biotransforming or biodegrading microbes based on chemotaxis. Pseudomonas aeruginosa strain KKc3, Ochrobactrum sp. strain KKc4, Achromobacter xylosoxidans strain KKc6 and Bacillus megaterium KKc7 were isolated based on their migration towards endosulfan in a soil column. Out of the four bacteria, B. megaterium converted endosulfan into toxic metabolite endosulfan sulphate, while the other three bacteria followed the non-toxic endosulfan diol pathway. The mixed culture system consisting of P. aeruginosa, Ochrobactrum sp and A. xylosoxidans could remove 94% of total endosulfan by using endosulfan as the sole source of sulphur.