Urinary proteins detected using modern proteomics intervene in early type 2 diabetic kidney disease - a pilot study

被引：9
|
作者：
Golea-Secara, Alina [1 ,2 ]
Munteanu, Cristian [3 ]
Sarbu, Mirela [4 ]
Cretu, Octavian M. [2 ,5 ]
Velciov, Silvia [1 ,2 ]
Vlad, Adrian [2 ,6 ]
Bob, Flaviu [1 ,2 ]
Gadalean, Florica [1 ,2 ]
Gluhovschi, Cristina [2 ]
Milas, Oana [1 ,2 ]
Simulescu, Anca [1 ,2 ]
Mogos-Stefan, Maria [1 ,2 ]
Patruica, Mihaela [1 ,2 ]
Petrica, Ligia [1 ,2 ,7 ]
Zamfir, Alina D. [4 ]
机构：
[1] Cty Emergency Hosp Timisoara, Dept Nephrol, Timisoara, Romania
[2] Victor Babes Univ Med & Pharm, Timisoara, Romania
[3] Inst Biochem, Dept Bioinformat & Struct Biochem, Bucharest, Romania
[4] Natl Inst Res & Dev Electrochem & Condensed Matte, Timisoara, Romania
[5] Municipal Emergency Hosp Timisoara, Dept Surg 1, Timisoara, Romania
[6] Cty Emergency Hosp, Dept Diabet & Metab Dis, Timisoara, Romania
[7] Victor Babes Univ Med & Pharm, Ctr Translat Res & Syst Med, Timisoara, Romania
来源：
BIOMARKERS IN MEDICINE | 2020年 / 14卷 / 16期
关键词：
albuminuria; diabetes mellitus Type 2; inflammation; proximal tubule dysfunction; urinary proteomics; BIOMARKERS; IDENTIFICATION; NEPHROPATHY; PATHOPHYSIOLOGY; EXPRESSION; PEPTIDES;
D O I：
10.2217/bmm-2020-0308
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aim:An advanced proteomics platform for protein biomarker discovery in diabetic chronic kidney disease (DKD) was developed, validated and implemented.Materials & methods:Three Type 2 diabetes mellitus patients and three control subjects were enrolled. Urinary peptides were extracted, samples were analyzed on a hybrid LTQ-Orbitrap Velos Pro instrument. Raw data were searched using the SEQUEST algorithm and integrated into Proteome Discoverer platform.Results & discussion:Unique peptide sequences, resulted sequence coverage, scoring of peptide spectrum matches were reported to albuminuria and databases. Five proteins that can be associated with early DKD were found: apolipoprotein AI, neutrophil gelatinase-associated lipocalin, cytidine deaminase, S100-A8 and hemoglobin subunit delta.Conclusion:Urinary proteome analysis could be used to evaluate mechanisms of pathogenesis of DKD.
引用
收藏
页码：1521 / 1537
页数：17
相关论文
共 50 条
  • [21] Association of Diabetic Kidney Disease Markers and Urinary Beta-crossLaps in Type 2 Diabetes
    Arumugasamy, Lalithambigai
    Patel, Hetal G.
    JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2021, 15 (07)
  • [22] Rationale and design of the Innsbruck Diabetic Kidney Disease Cohort (IDKDC)-a prospective study investigating etiology and progression of early-stage chronic kidney disease in type 2 diabetes
    Plattner, Clemens
    Sallaberger, Sebastian
    Bohn, Jan-Paul
    Zavadil, Claudia
    Keller, Felix
    Soleiman, Afschin
    Tiefenthaler, Martin
    Mayer, Gert
    Pirklbauer, Markus
    CLINICAL KIDNEY JOURNAL, 2024, 17 (05)
  • [23] Urine inositol pentakisphosphate 2-kinase and changes in kidney structure in early diabetic kidney disease in type 1 diabetes
    Looker, Helen C.
    Merchant, Michael L.
    Rane, Madhavi J.
    Nelson, Robert G.
    Kimmel, Paul L.
    Rovin, Brad H.
    Klein, Jon B.
    Mauer, Michael
    AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2018, 315 (05) : F1484 - F1492
  • [24] Serum Cystatin C as an Early Diagnostic Biomarker of Diabetic Kidney Disease in Type 2 Diabetic Patients
    Qamar, Ayesha
    Hayat, Asma
    Ahmad, Tariq Mahmood
    Khan, Alamgir
    ul Hasnat, Mohammad Najam
    Tahir, Sufyan
    JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN, 2018, 28 (04): : 288 - 291
  • [25] Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease
    Satirapoj, Bancha
    Dispan, Rattanawan
    Radinahamed, Piyanuch
    Kitiyakara, Chagriya
    BMC NEPHROLOGY, 2018, 19
  • [26] Diabetic kidney disease in patients with type 2 diabetes mellitus: a cross-sectional study
    Farah, Randa I.
    Al-Sabbagh, Mohammed Q.
    Momani, Munther S.
    Albtoosh, Asma
    Arabiat, Majd
    Abdulraheem, Ahmad M.
    Aljabiri, Husam
    Abufaraj, Mohammad
    BMC NEPHROLOGY, 2021, 22 (01)
  • [27] A Lifestyle Intervention to Delay Early Chronic Kidney Disease in African Americans With Diabetic Kidney Disease: Pre-Post Pilot Study
    Ozieh, Mukoso N.
    Egede, Leonard E.
    JMIR FORMATIVE RESEARCH, 2022, 6 (03)
  • [28] Relation between early stages of diabetic retinopathy and early stages of diabetic kidney disease in patients with type 2 diabetes in Egypt
    Lachine, Nagwa Amr
    Moez, Pacint Elsayed
    Rohoma, Kamel Hemida
    Abd El Latif, Eiman Mahmoud
    Kassab, Heba Sadek
    CLINICAL DIABETOLOGY, 2019, 8 (03): : 154 - 160
  • [29] Urinary and Serum Amino Acids May Be Associated with Podocyte, Proximal Tubule, and Renal Endothelial Injury in Early Diabetic Kidney Disease in Type 2 Diabetes Mellitus Patients
    Mogos, Maria
    Milas, Oana
    Socaciu, Carmen
    Socaciu, Andreea Iulia
    Vlad, Adrian
    Gadalean, Florica
    Bob, Flaviu
    Cretu, Octavian Marius
    Suteanu-Simulescu, Anca
    Glavan, Mihaela
    Balint, Lavinia
    Ienciu, Silvia
    Iancu, Iuliana-Lavinia
    Jianu, Dragos Catalin
    Ursoniu, Sorin
    Petrica, Ligia
    BIOMEDICINES, 2025, 13 (03)
  • [30] Urinary metabolites associate with the presence of diabetic kidney disease in type 2 diabetes and mediate the effect of inflammation on kidney complication
    Caifeng Shi
    Yemeng Wan
    Aiqin He
    Xiaomei Wu
    Xinjia Shen
    Xueting Zhu
    Junwei Yang
    Yang Zhou
    Acta Diabetologica, 2023, 60 : 1199 - 1207
12345