Glioblastoma U-87MG tumour cells suppressed by ZnO folic acid-conjugated nanoparticles: an in vitro study

被引:24
作者
Marfavi, Zahra Hamod [1 ]
Farhadi, Mona [1 ]
Jameie, Seyed Behnamedin [2 ,3 ]
Zahmatkeshan, Masoomeh [2 ]
Pirhajati, Vahid [2 ]
Jameie, Manasadat [2 ,4 ]
机构
[1] Islamic Azad Univ, Karaj Branch, Dept Microbiol, POB 31485-313, Karaj, Iran
[2] Iran Univ Med Sci, NRC, Tehran, Iran
[3] Iran Univ Med Sci, Fac Allied Med, Dept Med Basic Sci, Tehran, Iran
[4] Shahid Beheshti Univ Med Sci, Dept Anat, Fac Med, Tehran, Iran
关键词
Conjugated nanoparticles; zinc oxide; folic acid; glioblastoma; ROS; OXIDE NANOPARTICLES; RADIATION-THERAPY; CANCER-CELLS; APOPTOSIS;
D O I
10.1080/21691401.2019.1577889
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Glioblastoma Multiform (GBM) known as the most common CNS malignant tumour. Therapy for GBM includes maximum tumour resection and chemotherapy. Recent advances have emphasized the use of nanoparticles, such as zinc oxide nanoparticles (ZnO-NPs). Conjugated ZnO NPs with folic acid (FA) easily pass through cell membrane. In the present study, ZnO NPs-FA applied to GBM U87MG cell line. ZnO NPs-FA synthesized according to the sol-gel method. The GBM U87MG and astrocytes 1321N1 cell lines cultured and divided into control, sham and ZnO NPs-FA groups. MTT assay used for the cell viability, and ROS assay and flow cytometry exploited. The size of nanoparticles was <= 20 nm using TEM and FTIR. After 12 hours, the viability for U87MG cells showed a significant decrease at 1.25 and 2.5 mg/ml concentrations. However, no such results obtained for astrocytes. According to the results, the ROS assay caused a significant increase in GBM cells at the mentioned concentration. It was concluded that dose-dependent conjugated NPs could play a therapeutic role in cancer therapy. [GRAPHICS] .
引用
收藏
页码:2783 / 2790
页数:8
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