Carica papaya lipase (CPL) stored in the crude papain is found to be enantioselective for the kinetic resolution of (RS)-2-(4-chlorophenoxy)propionic acid via esterification in anhydrous organic solvents. Of the alcohols screened, trimethylsilylmethanol acted as the best acyl acceptor and gave the highest enzyme activity and enantioselectivity in anhydrous cyclohexane. The kinetic analysis at temperatures between 20 and 60 degreesC indicated that CPL is thermally stable, giving a high E value of 113 at 20 degreesC. A change of cyclohexane to other hydrophobic solvents resulted in better lipase performances. In comparison with the performances of other crude Candida rugosa lipases, Carica papaya lipase is more active, enantioselective and thermally stable. (C) 2004 Elsevier Ltd. All rights reserved.
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Univ Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, MalaysiaUniv Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, Malaysia
Sarijo, S. H.
Ahmad, A.
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Univ Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, MalaysiaUniv Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, Malaysia
Ahmad, A.
Muhsin, S. M. N.
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Malaysian Palm Oil Board, Persiaran Inst, 6 Bandar Baru Bangi, Kajang 43000, Selangor, MalaysiaUniv Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, Malaysia
Muhsin, S. M. N.
Jubri, Z.
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Univ Tenaga Nas, Coll Engn, Dept Engn Sci & Math, Kajang 43000, Selangor, MalaysiaUniv Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, Malaysia
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KYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPANKYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPAN
KAWAMOTO, T
SONOMOTO, K
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KYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPANKYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPAN
SONOMOTO, K
TANAKA, A
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KYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPANKYOTO UNIV, FAC ENGN,DEPT IND CHEM,IND BIOCHEM LAB,SANKYO KU, KYOTO 606, JAPAN
机构:
Scott Christian Coll Autonomous, Dept Phys, Nagercoil 629003, Tamil Nadu, IndiaScott Christian Coll Autonomous, Dept Phys, Nagercoil 629003, Tamil Nadu, India
Monicka, J. Clemy
James, C.
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Scott Christian Coll Autonomous, Dept Phys, Nagercoil 629003, Tamil Nadu, IndiaScott Christian Coll Autonomous, Dept Phys, Nagercoil 629003, Tamil Nadu, India