Preliminary X-ray crystallographic studies of the TIR domain of human Toll-like receptor 6

Toll-like receptor (TLR) proteins have been identified and shown to play a role in the innate immune response. TLR6 associated with TLR2 can recognize diacylated lipoprotein. In this study, the human TLR6 TIR domain corresponding to amino acids 640-796 was overexpressed in Escherichia coli using engineered C-terminal His tags. The TLR6 TIR domain was then purified to homogeneity and crystallized at 20 degrees C. Finally, X-ray diffraction data were collected to a resolution of 2.2 angstrom from a crystal belonging to space group C2, with unit-cell parameters a = 127.60, b = 44.20, c = 75.72 angstrom beta = 118.89 degrees