Down-regulation of Gli-1 inhibits hepatocellular carcinoma cell migration and invasion

被引：47

作者：

Chen, Jing-Song ^{[1
]}

Li, Hua-Shu ^{[2
]}

Huang, Jiong-Qiang ^{[1
]}

Zhang, Long-Juan ^{[3
]}

Chen, Xi-Lin ^{[4
]}

Wang, Qian ^{[4
]}

Lei, Jian ^{[1
]}

Feng, Ju-Tao ^{[1
]}

Liu, Qin ^{[1
]}

Huang, Xiao-Hui ^{[3
]}

机构：

[1] Guangzhou Med Univ, Affiliated Hosp 1, Dept Gastrointesinal Surg, Guangzhou 510120, Guangdong, Peoples R China

[2] Second Peoples Hosp Liwan Dist, Dept Gynaecol & Obstet, Guangzhou 510160, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Affiliated Hosp 1, Surg Lab, Guangzhou 510080, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Hepatobiliary Surg, Guangzhou 510080, Guangdong, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2014年 / 393卷 / 1-2期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Epithelial-mesenchymal transition; Glioma-associated oncogene homolog-1; Hepatocellular carcinoma; Invasion; Matrix metalloproteinase; EPITHELIAL-MESENCHYMAL TRANSITION; HEDGEHOG SIGNALING PATHWAY; TRANSCRIPTION FACTOR SNAIL; SONIC-HEDGEHOG; E-CADHERIN; MATRIX METALLOPROTEINASES; PROGNOSTIC MARKERS; BREAST-CANCER; EXPRESSION; METASTASIS;

D O I：

10.1007/s11010-014-2071-x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Glioma-associated oncogene homolog-1 (Gli-1) is considered a marker of Hedgehog pathway activation and is associated with the progression of several cancers. We have previously reported that Gli-1 was correlated with invasion and metastasis in hepatocellular carcinoma (HCC). However, the exact roles and mechanisms of Gli-1 in HCC invasion are unclear. In this study, we found that small interfering RNA mediated down-regulation of Gli-1 expression significantly suppressed adhesion, motility, migration, and invasion of both SMMC-7721 and SK-Hep1 cells. Furthermore, down-regulation of Gli-1 significantly reduced expressions and activities of both matrix metalloproteinase (MMP)-2 and MMP-9. In addition, we found that down-regulation of Gli-1 resulted in up-regulation of E-cadherin and concomitant down-regulation of Snail and Vimentin, consistent with inhibition of epithelial-mesenchymal transition (EMT). Taken together, our results suggest that down-regulation of Gli-1 suppresses HCC cell migration and invasion likely through inhibiting expressions and activations of MMP-2, 9 and blocking EMT.

引用

页码：283 / 291

页数：9

共 39 条

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