AIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling

被引：20

作者：

Brasil, Juliana Nogueira ^{[1
]}

Cabral, Luiz Mors ^{[2
]}

Eloy, Nubia B. ^{[3
]}

Primo, Luiza M. F. ^{[1
,4
]}

Barroso-Neto, Ito Liberato ^{[5
]}

Perdigao Grangeiro, Leticia P. ^{[1
]}

Gonzalez, Nathalie ^{[3
]}

Inze, Dirk ^{[3
]}

Ferreira, Paulo C. G. ^{[1
]}

Hemerly, Adriana S. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil

[2] Univ Fed Fluminense, Dept Biol Celular & Mol, Rio De Janeiro, Brazil

[3] VIB, Dept Plant Syst Biol, Ghent, Belgium

[4] Inst Nacl Canc, Programa Biol Celular, Rio De Janeiro, Brazil

[5] Univ Fed Ceara, Dept Quim, Fortaleza, Ceara, Brazil

来源：

BMC PLANT BIOLOGY | 2015年 / 15卷

关键词：

Agenet/Tudor; Tudor; DUF7; DUF724; ABAP1; Chromatin remodeling; Cell cycle; Arabidopsis; FLOWER DEVELOPMENT; GENE ACTIVITY; TUDOR DOMAIN; METHYLATION; BINDING; RNA; SEQUENCE; ENCODES; DEFENSE; PLANTS;

D O I：

10.1186/s12870-015-0641-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: DNA replication and transcription are dynamic processes regulating plant development that are dependent on the chromatin accessibility. Proteins belonging to the Agenet/Tudor domain family are known as histone modification "readers" and classified as chromatin remodeling proteins. Histone modifications and chromatin remodeling have profound effects on gene expression as well as on DNA replication, but how these processes are integrated has not been completely elucidated. It is clear that members of the Agenet/Tudor family are important regulators of development playing roles not well known in plants. Methods: Bioinformatics and phylogenetic analyses of the Agenet/Tudor Family domain in the plant kingdom were carried out with sequences from available complete genomes databases. 3D structure predictions of Agenet/Tudor domains were calculated by I-TASSER server. Protein interactions were tested in two-hybrid, GST pulldown, semi-in vivo pulldown and Tandem Affinity Purification assays. Gene function was studied in a T-DNA insertion GABI-line. Results: In the present work we analyzed the family of Agenet/Tudor domain proteins in the plant kingdom and we mapped the organization of this family throughout plant evolution. Furthermore, we characterized a member from Arabidopsis thaliana named AIP1 that harbors Agenet/Tudor and DUF724 domains. AIP1 interacts with ABAP1, a plant regulator of DNA replication licensing and gene transcription, with a plant histone modification "reader" (LHP1) and with non modified histones. AIP1 is expressed in reproductive tissues and its down-regulation delays flower development timing. Also, expression of ABAP1 and LHP1 target genes were repressed in flower buds of plants with reduced levels of AIP1. Conclusions: AIP1 is a novel Agenet/Tudor domain protein in plants that could act as a link between DNA replication, transcription and chromatin remodeling during flower development.

引用

页数：21

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