Enhancing the thermostability of α-L- rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol

被引：43

作者：

Ge, Lin ^{[1
,2
]}

Chen, Anna ^{[1
,2
]}

Pei, Jianjun ^{[1
,2
]}

Zhao, Linguo ^{[1
,2
]}

Fang, Xianying ^{[1
,2
]}

Ding, Gang ^{[3
]}

Wang, Zhenzhong ^{[3
]}

Xiao, Wei ^{[3
]}

Tang, Feng ^{[4
]}

机构：

[1] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, 159 Long Pan Rd, Nanjing 210037, Jiangsu, Peoples R China

[2] Nanjing Forestry Univ, Coll Chem Engn, 159 Long Pan Rd, Nanjing 210037, Jiangsu, Peoples R China

[3] Jiangsu Kanion Pharmaceut Co Ltd, 58 Haichang South Rd, Lianyungang 222001, Jiangsu, Peoples R China

[4] Int Ctr Bamboo & Rattan, 8 FuTong East St, Beijing 100714, Peoples R China

来源：

BMC BIOTECHNOLOGY | 2017年 / 17卷

基金：

中国国家自然科学基金;

关键词：

Thermostability; alpha-L-Rhamnosidase; Sorbitol; Enzymatic conversion; WEAKLY INTERACTING COSOLVENTS; FAGOPYRUM-ESCULENTUM; BETA-CYCLODEXTRIN; PURIFICATION; NARINGINASE; STABILITY; STABILIZATION; IMPROVEMENT; EXPRESSION; NIGER;

D O I：

10.1186/s12896-017-0342-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Thermally stable alpha-L- rhamnosidase with cleaving terminal alpha-L- rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability of alpha-L- rhamnosidase. Results: In this study, addition of sorbitol has been found to increase the thermostability of alpha-L- rhamnosidase from Aspergillus terreus at temperatures ranging from 65 degrees C to 80 degrees C. Half-life and activation free energy with addition of 2.0 M sorbitol at 70 degrees C were increased by 17.2-fold, 8.2 kJ/ mol, respectively. The analyses of the results of fluorescence spectroscopy and CD have indicated that sorbitol helped to protect the tertiary and secondary structure of alpha-L- rhamnosidase. Moreover, the isoquercitrin yield increased from 60.01 to 96.43% with the addition of 1.5 M of sorbitol at 70 degrees C. Conclusion: Our findings provide an effective approach for enhancing the thermostability of alpha-L-rhamnosidase from Aspergillus terreus, which makes it a good candidate for industrial processes of isoquercitrin preparation.

引用

页数：10

共 54 条

[1] Isoquercitrin isolated from Hyptis fasciculata reduces glioblastoma cell proliferation and changes β-catenin cellular localization [J].