Proteomic analysis of tissue samples in translational breast cancer research

被引：8
作者：
Gromov, Pavel [1 ,2 ]
Moreira, Jose M. A. [2 ,3 ,4 ]
Gromova, Irina [1 ,2 ]
机构：
[1] Danish Canc Soc Res Ctr, DK-2100 Copenhagen, Denmark
[2] Danish Ctr Translat Breast Canc Res DCTB, DK-2100 Copenhagen, Denmark
[3] Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet Dis Biol, Sect Mol Dis Biol, DK-1870 Frederiksberg, Denmark
[4] Univ Copenhagen, Sino Danish Breast Canc Res Ctr, DK-1870 Frederiksberg, Denmark
来源：
EXPERT REVIEW OF PROTEOMICS | 2014年 / 11卷 / 03期
关键词：
breast cancer; gel-based proteomics; immunohistochemistry; peptide-centric proteomics; protein arrays; tumor heterogeneity; LASER CAPTURE MICRODISSECTION; IMAGING MASS-SPECTROMETRY; PARAFFIN-EMBEDDED TISSUE; DIFFERENTIALLY EXPRESSED PROTEINS; INTERSTITIAL FLUID; TUMOR MICROENVIRONMENT; BIOMARKER DISCOVERY; DUCTAL CARCINOMA; GENETIC-HETEROGENEITY; THERAPEUTIC TARGET;
D O I：
10.1586/14789450.2014.899469
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In the last decade, many proteomic technologies have been applied, with varying success, to the study of tissue samples of breast carcinoma for protein expression profiling in order to discover protein biomarkers/signatures suitable for: characterization and subtyping of tumors; early diagnosis, and both prognosis and prediction of outcome of chemotherapy. The purpose of this review is to critically appraise what has been achieved to date using proteomic technologies and to bring forward novel strategies - based on the analysis of clinically relevant samples - that promise to accelerate the translation of basic discoveries into the daily breast cancer clinical practice. In particular, we address major issues in experimental design by reviewing the strengths and weaknesses of current proteomic strategies in the context of the analysis of human breast tissue specimens.
引用
收藏
页码：285 / 302
页数：18
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